Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 33104..34014 Replicon plasmid unnamed1
Accession NZ_CP054968
Organism Klebsiella pneumoniae strain STIN_94

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag HUZ62_RS25590 Protein ID WP_004026354.1
Coordinates 33544..34014 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag HUZ62_RS25585 Protein ID WP_004181895.1
Coordinates 33104..33547 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ62_RS25565 (HUZ62_25470) 28259..28966 + 708 WP_004181864.1 hypothetical protein -
HUZ62_RS25570 (HUZ62_25475) 28971..30002 + 1032 WP_029505552.1 hypothetical protein -
HUZ62_RS25575 (HUZ62_25480) 31310..31732 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
HUZ62_RS25580 (HUZ62_25485) 31732..33003 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
HUZ62_RS25585 (HUZ62_25490) 33104..33547 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
HUZ62_RS25590 (HUZ62_25495) 33544..34014 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
HUZ62_RS25595 (HUZ62_25500) 34124..34384 - 261 WP_004026352.1 hypothetical protein -
HUZ62_RS25600 (HUZ62_25505) 34955..36136 - 1182 WP_239611782.1 IS481 family transposase -
HUZ62_RS25605 (HUZ62_25510) 36208..36366 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
HUZ62_RS25610 (HUZ62_25515) 36969..37943 + 975 WP_014386572.1 hypothetical protein -
HUZ62_RS25615 (HUZ62_25520) 38141..38518 - 378 WP_004181901.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / dfrA12 / aadA2 / qacE / sul1 / qnrB4 / blaDHA-1 / mph(A) pla 1..350285 350285
- flank IS/Tn - - 34955..36136 1181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T160749 WP_004026354.1 NZ_CP054968:33544-34014 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T160749 NZ_CP070906:2041518-2041621 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT160749 WP_004181895.1 NZ_CP054968:33104-33547 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT160749 NZ_CP070906:c2041659-2041388 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References