160745

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4541220..4542030 Replicon chromosome
Accession NZ_CP054967
Organism Klebsiella pneumoniae strain STIN_94

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag HUZ62_RS22140 Protein ID WP_002887280.1
Coordinates 4541220..4541753 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HUZ62_RS22145 Protein ID WP_002887278.1
Coordinates 4541764..4542030 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ62_RS22135 (4540052) 4540052..4541173 + 1122 WP_009309849.1 cupin domain-containing protein -
HUZ62_RS22140 (4541220) 4541220..4541753 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
HUZ62_RS22145 (4541764) 4541764..4542030 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HUZ62_RS22150 (4542133) 4542133..4543566 - 1434 WP_023288193.1 Cu(+)/Ag(+) sensor histidine kinase -
HUZ62_RS22155 (4543556) 4543556..4544239 - 684 WP_032105399.1 copper response regulator transcription factor CusR -
HUZ62_RS22160 (4544412) 4544412..4545797 + 1386 WP_032431458.1 efflux transporter outer membrane subunit -
HUZ62_RS22165 (4545815) 4545815..4546120 + 306 WP_032432083.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T160745 WP_002887280.1 NZ_CP054967:c4541753-4541220 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T160745 NZ_CP070906:1058707-1059042 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT160745 WP_002887278.1 NZ_CP054967:c4542030-4541764 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT160745 NZ_CP070906:1058459-1058707 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGTTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLZ1

References