Detailed information of TA system TA160734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1433783..1434519
• Replicon: chromosome
• Accession: NZ_CP054967
• Organism: Klebsiella pneumoniae strain STIN_94

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A4S4Y1H2
• Locus tag: HUZ62_RS07030
• Protein ID: WP_032433360.1
• Coordinates: 1434037..1434519 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HUZ62_RS07025
• Protein ID: WP_003026799.1
• Coordinates: 1433783..1434049 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ62_RS07000 (1429429)	1429429..1430568	+	1140	WP_032433368.1	mannitol dehydrogenase	-
HUZ62_RS07005 (1430597)	1430597..1431259	+	663	WP_032433366.1	dihydroxyacetone kinase subunit DhaL	-
HUZ62_RS07010 (1431243)	1431243..1432247	+	1005	WP_032433364.1	dihydroxyacetone kinase subunit DhaK	-
HUZ62_RS07015 (1432265)	1432265..1432897	+	633	WP_032433362.1	3-hexulose-6-phosphate synthase	-
HUZ62_RS07020 (1432907)	1432907..1433470	+	564	WP_032433361.1	6-phospho-3-hexuloisomerase	-
HUZ62_RS07025 (1433783)	1433783..1434049	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HUZ62_RS07030 (1434037)	1434037..1434519	+	483	WP_032433360.1	GNAT family N-acetyltransferase	Toxin
HUZ62_RS07035 (1434719)	1434719..1436122	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
HUZ62_RS07040 (1436151)	1436151..1436783	-	633	WP_001567369.1	hypothetical protein	-
HUZ62_RS07045 (1437163)	1437163..1437762	-	600	WP_032432064.1	helix-turn-helix transcriptional regulator	-
HUZ62_RS07050 (1437975)	1437975..1438919	-	945	WP_077254249.1	fimbrial protein	-
HUZ62_RS07055 (1438931)	1438931..1439509	-	579	WP_032432061.1	type 1 fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1401728..1447745	46017
	-	flank	IS/Tn	-	-	1434719..1436122	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17421.02 Da
• Isoelectric Point: 9.6047

>T160734 WP_032433360.1 NZ_CP054967:1434037-1434519 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T160734 NZ_CP070902:3470040-3470258 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT160734 WP_003026799.1 NZ_CP054967:1433783-1434049 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT160734 NZ_CP070902:3469640-3470014 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A4S4Y1H2

Antitoxin

• AlphaFold DB: A0A1Q8YL66