Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 75833..76476 Replicon plasmid unnamed1
Accession NZ_CP054966
Organism Escherichia coli strain STIN_95

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag HUZ63_RS24905 Protein ID WP_001034044.1
Coordinates 76060..76476 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag HUZ63_RS24900 Protein ID WP_001261286.1
Coordinates 75833..76063 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ63_RS24885 (HUZ63_24630) 70970..71200 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
HUZ63_RS24890 (HUZ63_24635) 71197..71613 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
HUZ63_RS24895 (HUZ63_24640) 71658..75452 - 3795 WP_001144732.1 hypothetical protein -
HUZ63_RS24900 (HUZ63_24645) 75833..76063 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ63_RS24905 (HUZ63_24650) 76060..76476 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ63_RS24910 (HUZ63_24655) 76551..78116 + 1566 WP_001128474.1 AAA family ATPase -
HUZ63_RS24915 (HUZ63_24660) 78101..79123 + 1023 WP_000361402.1 helicase UvrD -
HUZ63_RS24925 (HUZ63_24670) 80130..80288 - 159 Protein_92 fluoride efflux transporter CrcB -
HUZ63_RS24930 (HUZ63_24675) 80629..81042 - 414 Protein_93 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / catA1 / aph(3')-Ia / blaTEM-1B / sitABCD - 1..87961 87961


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T160729 WP_001034044.1 NZ_CP054966:76060-76476 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T160729 NZ_CP070902:1799546-1799800 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT160729 WP_001261286.1 NZ_CP054966:75833-76063 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT160729 NZ_CP070902:1799298-1799549 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References