Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 70970..71613 Replicon plasmid unnamed1
Accession NZ_CP054966
Organism Escherichia coli strain STIN_95

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag HUZ63_RS24890 Protein ID WP_001034046.1
Coordinates 71197..71613 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag HUZ63_RS24885 Protein ID WP_001261278.1
Coordinates 70970..71200 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ63_RS24855 (HUZ63_24600) 66481..66786 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
HUZ63_RS24860 (HUZ63_24605) 66788..67006 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
HUZ63_RS24865 (HUZ63_24610) 67597..68085 + 489 WP_011254646.1 hypothetical protein -
HUZ63_RS24870 (HUZ63_24615) 68119..69252 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
HUZ63_RS24875 (HUZ63_24620) 69419..70192 - 774 WP_000905949.1 hypothetical protein -
HUZ63_RS24880 (HUZ63_24625) 70205..70705 - 501 WP_000528931.1 HEPN family nuclease -
HUZ63_RS24885 (HUZ63_24630) 70970..71200 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ63_RS24890 (HUZ63_24635) 71197..71613 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ63_RS24895 (HUZ63_24640) 71658..75452 - 3795 WP_001144732.1 hypothetical protein -
HUZ63_RS24900 (HUZ63_24645) 75833..76063 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
HUZ63_RS24905 (HUZ63_24650) 76060..76476 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / catA1 / aph(3')-Ia / blaTEM-1B / sitABCD - 1..87961 87961


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T160728 WP_001034046.1 NZ_CP054966:71197-71613 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T160728 NZ_CP070902:1799298-1799549 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT160728 WP_001261278.1 NZ_CP054966:70970-71200 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT160728 NZ_CP070902:1799546-1799800 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References