Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 5213..5772 Replicon plasmid unnamed1
Accession NZ_CP054966
Organism Escherichia coli strain STIN_95

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag HUZ63_RS24500 Protein ID WP_000421272.1
Coordinates 5213..5488 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag HUZ63_RS24505 Protein ID WP_001387467.1
Coordinates 5488..5772 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ63_RS24465 (HUZ63_24235) 1263..2003 - 741 WP_001066953.1 tyrosine-type recombinase/integrase -
HUZ63_RS24470 (HUZ63_24240) 2124..2306 - 183 WP_042344623.1 hypothetical protein -
HUZ63_RS24475 2373..2662 - 290 Protein_3 type II toxin-antitoxin system RelE/ParE family toxin -
HUZ63_RS24480 (HUZ63_24245) 2649..2917 - 269 Protein_4 type II toxin-antitoxin system ParD family antitoxin -
HUZ63_RS24485 (HUZ63_24250) 3449..4618 + 1170 WP_001072355.1 hypothetical protein -
HUZ63_RS24490 (HUZ63_24255) 4651..4779 + 129 Protein_6 IS481 family transposase -
HUZ63_RS24495 (HUZ63_24260) 4904..5107 - 204 Protein_7 hypothetical protein -
HUZ63_RS24500 (HUZ63_24265) 5213..5488 - 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUZ63_RS24505 (HUZ63_24270) 5488..5772 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
HUZ63_RS24510 (HUZ63_24275) 6377..7129 + 753 WP_000562172.1 IclR family transcriptional regulator -
HUZ63_RS24515 (HUZ63_24280) 7175..8071 - 897 WP_239634788.1 2-keto-3-deoxygluconate permease -
HUZ63_RS24520 (HUZ63_24285) 8096..8800 + 705 WP_001067834.1 IS6-like element IS26 family transposase -
HUZ63_RS24525 (HUZ63_24290) 8858..9388 - 531 Protein_13 histidine-type phosphatase -
HUZ63_RS24530 (HUZ63_24295) 9510..9995 - 486 WP_000874189.1 hypothetical protein -
HUZ63_RS24535 (HUZ63_24300) 10020..10505 - 486 WP_001267177.1 TlpA disulfide reductase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / catA1 / aph(3')-Ia / blaTEM-1B / sitABCD - 1..87961 87961
- flank IS/Tn - - 8096..8800 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T160726 WP_000421272.1 NZ_CP054966:c5488-5213 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T160726 NZ_CP070902:863773-864180 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT160726 WP_001387467.1 NZ_CP054966:c5772-5488 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT160726 NZ_CP070902:863526-863792 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References