Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4242560..4243136 Replicon chromosome
Accession NZ_CP054965
Organism Escherichia coli strain STIN_95

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag HUZ63_RS20545 Protein ID WP_001350372.1
Coordinates 4242849..4243136 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0G9FLJ7
Locus tag HUZ63_RS20540 Protein ID WP_000063155.1
Coordinates 4242560..4242862 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ63_RS20525 (4239088) 4239088..4241238 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
HUZ63_RS20530 (4241356) 4241356..4241559 + 204 WP_000467859.1 YbdD/YjiX family protein -
HUZ63_RS20535 (4241570) 4241570..4242526 + 957 WP_001350371.1 GTPase -
HUZ63_RS20540 (4242560) 4242560..4242862 - 303 WP_000063155.1 BrnA antitoxin family protein Antitoxin
HUZ63_RS20545 (4242849) 4242849..4243136 - 288 WP_001350372.1 BrnT family toxin Toxin
HUZ63_RS20550 (4243333) 4243333..4244499 + 1167 WP_000800824.1 restriction endonuclease -
HUZ63_RS20555 (4244563) 4244563..4246182 + 1620 WP_001029741.1 class I SAM-dependent DNA methyltransferase -
HUZ63_RS20560 (4246172) 4246172..4247503 + 1332 WP_000505211.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T160722 WP_001350372.1 NZ_CP054965:c4243136-4242849 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T160722 NZ_CP070902:198769-198876 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11572.05 Da        Isoelectric Point: 9.4646

>AT160722 WP_000063155.1 NZ_CP054965:c4242862-4242560 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT160722 NZ_CP070902:c198720-198657 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0G9FLJ7

References