Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4132091..4132641 Replicon chromosome
Accession NZ_CP054965
Organism Escherichia coli strain STIN_95

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HUZ63_RS20000 Protein ID WP_001160966.1
Coordinates 4132091..4132405 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HUZ63_RS20005 Protein ID WP_000125566.1
Coordinates 4132408..4132641 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ63_RS19975 (4127766) 4127766..4129052 + 1287 WP_239634632.1 peptidylprolyl isomerase SurA -
HUZ63_RS19980 (4129052) 4129052..4130041 + 990 WP_000241228.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HUZ63_RS19985 (4130038) 4130038..4130859 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HUZ63_RS19990 (4130862) 4130862..4131239 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HUZ63_RS19995 (4131246) 4131246..4132094 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HUZ63_RS20000 (4132091) 4132091..4132405 - 315 WP_001160966.1 CcdB family protein Toxin
HUZ63_RS20005 (4132408) 4132408..4132641 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HUZ63_RS20010 (4132727) 4132727..4133206 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
HUZ63_RS20015 (4133398) 4133398..4135260 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
HUZ63_RS20020 (4135253) 4135253..4135783 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HUZ63_RS20025 (4135891) 4135891..4137222 - 1332 WP_001183207.1 MFS transporter -
HUZ63_RS20030 (4137281) 4137281..4137568 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T160719 WP_001160966.1 NZ_CP054965:c4132405-4132091 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T160719 NZ_CP070899:c126049-125606 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT160719 WP_000125566.1 NZ_CP054965:c4132641-4132408 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT160719 NZ_CP070899:c126276-126046 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References