Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1160230..1160813 Replicon chromosome
Accession NZ_CP054965
Organism Escherichia coli strain STIN_95

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag HUZ63_RS05555 Protein ID WP_000254750.1
Coordinates 1160478..1160813 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag HUZ63_RS05550 Protein ID WP_000581937.1
Coordinates 1160230..1160478 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ63_RS05540 (1156569) 1156569..1157870 + 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
HUZ63_RS05545 (1157918) 1157918..1160152 + 2235 WP_239634718.1 GTP diphosphokinase -
HUZ63_RS05550 (1160230) 1160230..1160478 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HUZ63_RS05555 (1160478) 1160478..1160813 + 336 WP_000254750.1 endoribonuclease MazF Toxin
HUZ63_RS05560 (1160885) 1160885..1161676 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HUZ63_RS05565 (1161904) 1161904..1163541 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HUZ63_RS05570 (1163629) 1163629..1164927 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T160703 WP_000254750.1 NZ_CP054965:1160478-1160813 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T160703 NZ_CP070891:261137-261421 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTCCATGGACGCAAAGACCAGTACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTAACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTGACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT160703 WP_000581937.1 NZ_CP054965:1160230-1160478 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT160703 NZ_CP070891:260899-261147 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACCTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCATATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References