Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1006727..1007381 Replicon chromosome
Accession NZ_CP054965
Organism Escherichia coli strain STIN_95

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HUZ63_RS04940 Protein ID WP_000244781.1
Coordinates 1006974..1007381 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ63_RS04935 Protein ID WP_000354046.1
Coordinates 1006727..1006993 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ63_RS04915 (1002815) 1002815..1004248 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
HUZ63_RS04920 (1004293) 1004293..1004604 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
HUZ63_RS04925 (1004768) 1004768..1005427 + 660 WP_000250274.1 hemolysin III family protein -
HUZ63_RS04930 (1005504) 1005504..1006484 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
HUZ63_RS04935 (1006727) 1006727..1006993 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ63_RS04940 (1006974) 1006974..1007381 + 408 WP_000244781.1 protein YgfX Toxin
HUZ63_RS04945 (1007421) 1007421..1007942 - 522 WP_001055874.1 flavodoxin FldB -
HUZ63_RS04950 (1008054) 1008054..1008950 + 897 WP_000806633.1 site-specific tyrosine recombinase XerD -
HUZ63_RS04955 (1008975) 1008975..1009685 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ63_RS04960 (1009691) 1009691..1011424 + 1734 WP_000813193.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T160702 WP_000244781.1 NZ_CP054965:1006974-1007381 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T160702 NZ_CP070891:c156959-156516 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT160702 WP_000354046.1 NZ_CP054965:1006727-1006993 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT160702 NZ_CP070891:c157186-156956 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References