Detailed information of TA system TA160698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 943087..943646
• Replicon: chromosome
• Accession: NZ_CP054959
• Organism: Brucella suis bv.

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: HUZ28_RS04550
• Protein ID: WP_004688321.1
• Coordinates: 943401..943646 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: HUZ28_RS04545
• Protein ID: WP_002964097.1
• Coordinates: 943087..943401 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ28_RS04500	938777..938980	-	204	WP_004690812.1	hypothetical protein	-
HUZ28_RS04505	938983..939198	-	216	WP_002971459.1	hypothetical protein	-
HUZ28_RS04510	939195..939398	-	204	WP_002964088.1	hypothetical protein	-
HUZ28_RS04515	939448..940167	+	720	WP_006072696.1	hypothetical protein	-
HUZ28_RS04520	940164..940394	+	231	WP_002964091.1	hypothetical protein	-
HUZ28_RS04525	940391..940666	+	276	WP_002964092.1	hypothetical protein	-
HUZ28_RS04530	940689..941276	+	588	WP_004683738.1	hypothetical protein	-
HUZ28_RS04535	941511..942221	+	711	WP_004683739.1	porin family protein	-
HUZ28_RS04540	942569..942739	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
HUZ28_RS04545	943087..943401	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
HUZ28_RS04550	943401..943646	-	246	WP_004688321.1	BrnT family toxin	Toxin
HUZ28_RS04555	943779..944416	+	638	Protein_891	tRNA-guanine transglycosylase	-
HUZ28_RS04560	944486..945244	+	759	Protein_892	IS5 family transposase	-
HUZ28_RS04565	945245..946018	+	774	WP_006132649.1	hypothetical protein	-
HUZ28_RS04570	946153..947799	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	937005..951115	14110
	-	flank	IS/Tn	-	-	944486..944854	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T160698 WP_004688321.1 NZ_CP054959:c943646-943401 [Brucella suis bv. 1]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T160698 NZ_CP070890:c4801332-4801048 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT160698 WP_002964097.1 NZ_CP054959:c943401-943087 [Brucella suis bv. 1]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT160698 NZ_CP070890:c4801564-4801322 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA