Detailed information of TA system TA160697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 378355..378914
• Replicon: chromosome
• Accession: NZ_CP054957
• Organism: Brucella suis bv. 3 strain CVI_71

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: HUZ29_RS01715
• Protein ID: WP_004688321.1
• Coordinates: 378355..378600 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: HUZ29_RS01720
• Protein ID: WP_002964097.1
• Coordinates: 378600..378914 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ29_RS01695	374203..375849	-	1647	WP_004689673.1	hypothetical protein	-
HUZ29_RS01700	375984..376757	-	774	WP_006132649.1	hypothetical protein	-
HUZ29_RS01710	377585..378222	-	638	Protein_334	tRNA-guanine transglycosylase	-
HUZ29_RS01715	378355..378600	+	246	WP_004688321.1	BrnT family toxin	Toxin
HUZ29_RS01720	378600..378914	+	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
HUZ29_RS01725	379262..379432	+	171	WP_002964095.1	DUF1508 domain-containing protein	-
HUZ29_RS01730	379780..380490	-	711	WP_004683739.1	porin family protein	-
HUZ29_RS01735	380725..381312	-	588	WP_004683738.1	hypothetical protein	-
HUZ29_RS01740	381335..381610	-	276	WP_002964092.1	hypothetical protein	-
HUZ29_RS01745	381607..381837	-	231	WP_002964091.1	hypothetical protein	-
HUZ29_RS01750	381834..382553	-	720	WP_006072696.1	hypothetical protein	-
HUZ29_RS01755	382603..382806	+	204	WP_002964088.1	hypothetical protein	-
HUZ29_RS01760	382803..383018	+	216	WP_002971459.1	hypothetical protein	-
HUZ29_RS01765	383021..383224	+	204	WP_004690812.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wbdA / wboA	371089..384996	13907

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T160697 WP_004688321.1 NZ_CP054957:378355-378600 [Brucella suis bv. 3]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T160697 NZ_CP070890:c4691594-4691061 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT160697 WP_002964097.1 NZ_CP054957:378600-378914 [Brucella suis bv. 3]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT160697 NZ_CP070890:c4691871-4691605 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA