Detailed information of TA system TA160696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 942138..942697
• Replicon: chromosome
• Accession: NZ_CP054955
• Organism: Brucella suis bv. 4 strain CVI_72

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: HUZ30_RS04550
• Protein ID: WP_004688321.1
• Coordinates: 942452..942697 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: HUZ30_RS04545
• Protein ID: WP_002964097.1
• Coordinates: 942138..942452 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ30_RS04500	937828..938031	-	204	WP_004690812.1	hypothetical protein	-
HUZ30_RS04505	938034..938249	-	216	WP_002971459.1	hypothetical protein	-
HUZ30_RS04510	938246..938449	-	204	WP_002964088.1	hypothetical protein	-
HUZ30_RS04515	938499..939218	+	720	WP_006072696.1	hypothetical protein	-
HUZ30_RS04520	939215..939445	+	231	WP_002964091.1	hypothetical protein	-
HUZ30_RS04525	939442..939717	+	276	WP_002964092.1	hypothetical protein	-
HUZ30_RS04530	939740..940327	+	588	WP_004683738.1	hypothetical protein	-
HUZ30_RS04535	940562..941272	+	711	WP_004683739.1	porin family protein	-
HUZ30_RS04540	941620..941790	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
HUZ30_RS04545	942138..942452	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
HUZ30_RS04550	942452..942697	-	246	WP_004688321.1	BrnT family toxin	Toxin
HUZ30_RS04555	942830..943467	+	638	Protein_891	tRNA-guanine transglycosylase	-
HUZ30_RS04560	943537..944294	+	758	WP_080723468.1	IS5-like element IS711 family transposase	-
HUZ30_RS04565	944295..945068	+	774	WP_006132649.1	hypothetical protein	-
HUZ30_RS04570	945203..946849	+	1647	Protein_894	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	936056..950165	14109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T160696 WP_004688321.1 NZ_CP054955:c942697-942452 [Brucella suis bv. 4]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T160696 NZ_CP070890:4064036-4064254 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT160696 WP_002964097.1 NZ_CP054955:c942452-942138 [Brucella suis bv. 4]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT160696 NZ_CP070890:4063635-4064009 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG