Detailed information of TA system TA160694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 962131..962690
• Replicon: chromosome
• Accession: NZ_CP054945
• Organism: Brucella suis bv.

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: HUZ35_RS04665
• Protein ID: WP_004688321.1
• Coordinates: 962445..962690 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: HUZ35_RS04660
• Protein ID: WP_002964097.1
• Coordinates: 962131..962445 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ35_RS04615	957821..958024	-	204	WP_004690812.1	hypothetical protein	-
HUZ35_RS04620	958027..958242	-	216	WP_002971459.1	hypothetical protein	-
HUZ35_RS04625	958239..958442	-	204	WP_002964088.1	hypothetical protein	-
HUZ35_RS04630	958492..959211	+	720	WP_006072696.1	hypothetical protein	-
HUZ35_RS04635	959208..959438	+	231	WP_002964091.1	hypothetical protein	-
HUZ35_RS04640	959435..959710	+	276	WP_002964092.1	hypothetical protein	-
HUZ35_RS04645	959733..960320	+	588	WP_004683738.1	hypothetical protein	-
HUZ35_RS04650	960555..961265	+	711	WP_004683739.1	porin family protein	-
HUZ35_RS04655	961613..961783	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
HUZ35_RS04660	962131..962445	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
HUZ35_RS04665	962445..962690	-	246	WP_004688321.1	BrnT family toxin	Toxin
HUZ35_RS04670	962823..963460	+	638	Protein_915	tRNA-guanine transglycosylase	-
HUZ35_RS04675	963530..964287	+	758	WP_144313581.1	IS5 family transposase	-
HUZ35_RS04680	964288..965061	+	774	WP_006072701.1	hypothetical protein	-
HUZ35_RS04685	965196..966842	+	1647	WP_006072703.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	956037..970157	14120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T160694 WP_004688321.1 NZ_CP054945:c962690-962445 [Brucella suis bv. 2]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T160694 NZ_CP070890:1979450-1979552 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT160694 WP_002964097.1 NZ_CP054945:c962445-962131 [Brucella suis bv. 2]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT160694 NZ_CP070890:c1979559-1979414 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT