Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 136697..137340 Replicon plasmid pMS6192A-NDM
Accession NZ_CP054941
Organism Escherichia coli strain MS6192

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag MS6192_RS24425 Protein ID WP_001044768.1
Coordinates 136697..137113 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag MS6192_RS24430 Protein ID WP_001261287.1
Coordinates 137110..137340 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6192_RS24410 132001..132933 - 933 WP_000991832.1 hypothetical protein -
MS6192_RS24415 132937..133932 - 996 WP_000246636.1 hypothetical protein -
MS6192_RS24420 134397..136535 + 2139 WP_000350635.1 AAA family ATPase -
MS6192_RS24425 136697..137113 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
MS6192_RS24430 137110..137340 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MS6192_RS24435 137647..140766 + 3120 WP_001553851.1 hypothetical protein -
MS6192_RS24440 141029..142162 + 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaNDM-1 / sul1 / qacE / aadA2 / dfrA12 - 1..142890 142890
- flank IS/Tn - - 131361..131864 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T160685 WP_001044768.1 NZ_CP054941:c137113-136697 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T160685 NZ_CP070873:c3967641-3967387 [Escherichia fergusonii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACGGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACTCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT160685 WP_001261287.1 NZ_CP054941:c137340-137110 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT160685 NZ_CP070873:c3967889-3967638 [Escherichia fergusonii]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAACGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TATTGCGTTCCCCCGCTAATGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References