160684

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 124095..124696 Replicon plasmid pMS6192A-NDM
Accession NZ_CP054941
Organism Escherichia coli strain MS6192

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag MS6192_RS24380 Protein ID WP_001216034.1
Coordinates 124095..124475 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag MS6192_RS24385 Protein ID WP_001190712.1
Coordinates 124475..124696 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6192_RS24345 119146..120078 - 933 WP_000081352.1 homocysteine S-methyltransferase -
MS6192_RS24350 120065..121468 - 1404 WP_001373486.1 S-methylmethionine permease -
MS6192_RS24355 121676..122692 + 1017 WP_012372828.1 IS5-like element IS5 family transposase -
MS6192_RS24360 122920..123237 + 318 WP_001513659.1 hypothetical protein -
MS6192_RS24365 123187..123600 - 414 Protein_155 DDE-type integrase/transposase/recombinase -
MS6192_RS24370 123524..123883 - 360 WP_001513660.1 hypothetical protein -
MS6192_RS24375 123911..124090 - 180 WP_001513661.1 hypothetical protein -
MS6192_RS24380 124095..124475 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MS6192_RS24385 124475..124696 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MS6192_RS24390 124879..126435 + 1557 WP_001553856.1 type I restriction-modification system subunit M -
MS6192_RS24395 126432..127715 + 1284 WP_001553855.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaNDM-1 / sul1 / qacE / aadA2 / dfrA12 - 1..142890 142890


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T160684 WP_001216034.1 NZ_CP054941:c124475-124095 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T160684 NZ_CP070873:c3426394-3426116 [Escherichia fergusonii]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTATTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAAT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGTGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTTGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT160684 WP_001190712.1 NZ_CP054941:c124696-124475 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT160684 NZ_CP070873:c3426116-3425832 [Escherichia fergusonii]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAGCTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCCGCGTTGACGCCAGAAATGGCGA
TTAAACTTTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTACAAAACGCCTGGAGTCTGGCAGAGGCAGAA
AAAACGGTAGATGTGTCGCGACTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References