Detailed information of TA system TA160669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2770291..2770916
• Replicon: chromosome
• Accession: NZ_CP054940
• Organism: Escherichia coli strain MS6192

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MS6192_RS13520
• Protein ID: WP_000911330.1
• Coordinates: 2770291..2770689 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: MS6192_RS13525
• Protein ID: WP_000450524.1
• Coordinates: 2770689..2770916 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6192_RS13500	2766198..2766398	+	201	WP_000383836.1	YpfN family protein	-
MS6192_RS13505	2766479..2767177	-	699	WP_000679812.1	esterase	-
MS6192_RS13510	2767251..2769266	-	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
MS6192_RS13515	2769281..2770144	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
MS6192_RS13520	2770291..2770689	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
MS6192_RS13525	2770689..2770916	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
MS6192_RS13530	2771070..2771783	-	714	WP_001347624.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
MS6192_RS13535	2771996..2773030	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
MS6192_RS13540	2773047..2773925	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
MS6192_RS13545	2774071..2774643	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
MS6192_RS13550	2774643..2775113	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T160669 WP_000911330.1 NZ_CP054940:c2770689-2770291 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T160669 NZ_CP070850:14705-15049 [Lactiplantibacillus plantarum]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTGGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT160669 WP_000450524.1 NZ_CP054940:c2770916-2770689 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT160669 NZ_CP070850:14448-14711 [Lactiplantibacillus plantarum]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33