Detailed information of TA system TA160658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1684645..1686233
• Replicon: chromosome
• Accession: NZ_CP054940
• Organism: Escherichia coli strain MS6192

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: E0IX85
• Locus tag: MS6192_RS08085
• Protein ID: WP_001125454.1
• Coordinates: 1684645..1685967 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: MS6192_RS08090
• Protein ID: WP_001296726.1
• Coordinates: 1685967..1686233 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6192_RS08070	1682059..1682769	-	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-
MS6192_RS08075	1683130..1683693	-	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
MS6192_RS08080	1684125..1684333	-	209	Protein_1583	helix-turn-helix transcriptional regulator	-
MS6192_RS08085	1684645..1685967	-	1323	WP_001125454.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
MS6192_RS08090	1685967..1686233	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MS6192_RS08095	1686442..1687842	-	1401	WP_001083595.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	1671354..1686414	15060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49307.90 Da
• Isoelectric Point: 8.8753

>T160658 WP_001125454.1 NZ_CP054940:c1685967-1684645 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T160658 NZ_CP070610:997706-997966 [Rhodococcus koreensis]
ATGAAACTTGTCTGGGACGAGTCTGCGTGGGAGGACTACGTCTGGTGGCAGGCGCAGGATCGGAAGATCGTCAAGCGGAT
CGACACACTACTCAAGGATGTCGAACGCAACGGGAACGACGGAATCGGCAAGCCCGAGCCCCTCAAGCACGGTTTTCAGG
GCTACTGGTCCCGGCGTATCACCGATGAGCACCGGCTGGTTTATAAAATTGTCGATCATCAGATCCGCATTGCTGCCTGC
CGTTACCGCTACGGACACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT160658 WP_001296726.1 NZ_CP054940:c1686233-1685967 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT160658 NZ_CP070610:997410-997709 [Rhodococcus koreensis]
ATGTCGGGTTGCGTACAATTTCTTGTACGTAATAACGGAGGTGTGCTAAGGCGAACCATGACCTACTCGGAGTCTCGCGC
GCGGTACGCCGAGGTCCTCAACGCCGTCGTCGATGACCGGGAAGAAATCGTCATCACGCGGGCGGGTCACGAGCCGGTGG
TGATGGTTTCGCTCGACGACTATGAGTCGTTGAAGGAAACGGCATACTTGCTCAGGAGTCCGGAGAACGCCCGCCGGCTG
TTGGCGTCGATCGATCGGTTGGAGTCCGGCGGGGGAACTGTCCGGGATCTGGCTGAATGA

Structures

Toxin

• AlphaFold DB: E0IX85