Detailed information of TA system TA160657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1599909..1600547
• Replicon: chromosome
• Accession: NZ_CP054940
• Organism: Escherichia coli strain MS6192

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: MS6192_RS07725
• Protein ID: WP_000813794.1
• Coordinates: 1599909..1600085 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MS6192_RS07730
• Protein ID: WP_001270286.1
• Coordinates: 1600131..1600547 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6192_RS07705	1595528..1596742	-	1215	WP_001348055.1	BenE family transporter YdcO	-
MS6192_RS07710	1596795..1597331	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
MS6192_RS07715	1597404..1599365	+	1962	WP_001593323.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MS6192_RS07720	1599457..1599687	-	231	WP_000494244.1	YncJ family protein	-
MS6192_RS07725	1599909..1600085	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MS6192_RS07730	1600131..1600547	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MS6192_RS07735	1600626..1602032	+	1407	WP_000760592.1	PLP-dependent aminotransferase family protein	-
MS6192_RS07740	1602277..1603422	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
MS6192_RS07745	1603440..1604453	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
MS6192_RS07750	1604454..1605395	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1594340..1595488	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T160657 WP_000813794.1 NZ_CP054940:1599909-1600085 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T160657 NZ_CP070610:997410-997709 [Rhodococcus koreensis]
ATGTCGGGTTGCGTACAATTTCTTGTACGTAATAACGGAGGTGTGCTAAGGCGAACCATGACCTACTCGGAGTCTCGCGC
GCGGTACGCCGAGGTCCTCAACGCCGTCGTCGATGACCGGGAAGAAATCGTCATCACGCGGGCGGGTCACGAGCCGGTGG
TGATGGTTTCGCTCGACGACTATGAGTCGTTGAAGGAAACGGCATACTTGCTCAGGAGTCCGGAGAACGCCCGCCGGCTG
TTGGCGTCGATCGATCGGTTGGAGTCCGGCGGGGGAACTGTCCGGGATCTGGCTGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT160657 WP_001270286.1 NZ_CP054940:1600131-1600547 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT160657 NZ_CP070610:997706-997966 [Rhodococcus koreensis]
ATGAAACTTGTCTGGGACGAGTCTGCGTGGGAGGACTACGTCTGGTGGCAGGCGCAGGATCGGAAGATCGTCAAGCGGAT
CGACACACTACTCAAGGATGTCGAACGCAACGGGAACGACGGAATCGGCAAGCCCGAGCCCCTCAAGCACGGTTTTCAGG
GCTACTGGTCCCGGCGTATCACCGATGAGCACCGGCTGGTTTATAAAATTGTCGATCATCAGATCCGCATTGCTGCCTGC
CGTTACCGCTACGGACACTGA

Structures

Toxin

• PDB: 6HPB