160645

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 547999..548836 Replicon chromosome
Accession NZ_CP054940
Organism Escherichia coli strain MS6192

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag MS6192_RS02635 Protein ID WP_000227784.1
Coordinates 547999..548541 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag MS6192_RS02640 Protein ID WP_001297137.1
Coordinates 548525..548836 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6192_RS02615 543538..544449 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
MS6192_RS02620 544617..545108 + 492 WP_001138904.1 nucleotide binding protein YajQ -
MS6192_RS02625 545236..546600 - 1365 WP_001000974.1 MFS transporter -
MS6192_RS02630 547008..547943 + 936 WP_001365761.1 sel1 repeat family protein -
MS6192_RS02635 547999..548541 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
MS6192_RS02640 548525..548836 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
MS6192_RS02645 549021..549911 - 891 WP_000971336.1 heme o synthase -
MS6192_RS02650 549923..550252 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
MS6192_RS02655 550252..550866 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
MS6192_RS02660 550856..552847 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
MS6192_RS02665 552869..553816 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T160645 WP_000227784.1 NZ_CP054940:c548541-547999 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T160645 NZ_CP070602:c1245500-1245183 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT160645 WP_001297137.1 NZ_CP054940:c548836-548525 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT160645 NZ_CP070602:c1245190-1244903 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References