Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 312359..313038 Replicon chromosome
Accession NZ_CP054940
Organism Escherichia coli strain MS6192

Toxin (Protein)


Gene name ykfI Uniprot ID A0A2I8S2T2
Locus tag MS6192_RS01475 Protein ID WP_008324514.1
Coordinates 312359..312700 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A2I8S2U6
Locus tag MS6192_RS01480 Protein ID WP_008324516.1
Coordinates 312721..313038 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6192_RS01450 307636..308037 + 402 WP_000174677.1 sigma factor-binding protein Crl -
MS6192_RS01455 308076..309131 - 1056 WP_000749881.1 phosphoporin PhoE -
MS6192_RS01460 309419..310522 + 1104 WP_001285288.1 glutamate 5-kinase -
MS6192_RS01465 310534..311787 + 1254 WP_000893255.1 glutamate-5-semialdehyde dehydrogenase -
MS6192_RS01475 312359..312700 - 342 WP_008324514.1 type IV toxin-antitoxin system toxin YkfI Toxin
MS6192_RS01480 312721..313038 - 318 WP_008324516.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
MS6192_RS01485 313051..313278 - 228 WP_008324517.1 DUF987 domain-containing protein -
MS6192_RS01490 313287..313763 - 477 WP_000811693.1 RadC family protein -
MS6192_RS01495 313779..314237 - 459 WP_000211838.1 antirestriction protein -
MS6192_RS01500 314335..314574 - 240 WP_000194654.1 DUF905 domain-containing protein -
MS6192_RS01505 314651..315118 - 468 WP_001547765.1 hypothetical protein -
MS6192_RS01510 315141..315584 - 444 WP_008324520.1 hypothetical protein -
MS6192_RS01515 315584..315820 - 237 WP_001115854.1 hypothetical protein -
MS6192_RS01520 315857..316558 - 702 WP_008324530.1 WYL domain-containing protein -
MS6192_RS01525 316776..317597 - 822 WP_001548934.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 312359..322006 9647


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12919.98 Da        Isoelectric Point: 9.6543

>T160644 WP_008324514.1 NZ_CP054940:c312700-312359 [Escherichia coli]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T160644 NZ_CP070602:c69185-67863 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTTTCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCTACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11787.33 Da        Isoelectric Point: 7.3476

>AT160644 WP_008324516.1 NZ_CP054940:c313038-312721 [Escherichia coli]
MSNPTRGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Download         Length: 318 bp

>AT160644 NZ_CP070602:c69451-69185 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGATGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8S2T2

Antitoxin

Source ID Structure
AlphaFold DB A0A2I8S2U6

References