Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4325771..4326572 Replicon chromosome
Accession NZ_CP054913
Organism Shigella flexneri strain FDAARGOS_691

Toxin (Protein)


Gene name yeeV Uniprot ID D3GUT5
Locus tag FOB99_RS21630 Protein ID WP_001094430.1
Coordinates 4326195..4326572 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D3GUT4
Locus tag FOB99_RS21625 Protein ID WP_001285620.1
Coordinates 4325771..4326148 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB99_RS21595 4322392..4322847 + 456 WP_005051844.1 hypothetical protein -
FOB99_RS21600 4322865..4323079 + 215 Protein_4192 DUF905 family protein -
FOB99_RS21605 4323258..4324076 + 819 WP_001177758.1 DUF945 domain-containing protein -
FOB99_RS21610 4324418..4324891 + 474 WP_000855059.1 antirestriction protein -
FOB99_RS21615 4324907..4325383 + 477 WP_001387238.1 RadC family protein -
FOB99_RS21620 4325470..4325691 + 222 WP_000692350.1 DUF987 domain-containing protein -
FOB99_RS21625 4325771..4326148 + 378 WP_001285620.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
FOB99_RS21630 4326195..4326572 + 378 WP_001094430.1 TA system toxin CbtA family protein Toxin
FOB99_RS21635 4326569..4327057 + 489 WP_000761715.1 hypothetical protein -
FOB99_RS21640 4327077..4327274 + 198 WP_000772029.1 DUF957 domain-containing protein -
FOB99_RS21645 4327359..4328201 + 843 WP_001290187.1 DUF4942 domain-containing protein -
FOB99_RS21650 4328978..4329223 + 246 WP_000875412.1 hypothetical protein -
FOB99_RS21655 4329626..4330788 + 1163 WP_094081514.1 IS3 family transposase -
FOB99_RS21660 4330892..4331359 - 468 WP_011069513.1 prepilin peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4301128..4332812 31684


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13971.82 Da        Isoelectric Point: 6.8608

>T160627 WP_001094430.1 NZ_CP054913:4326195-4326572 [Shigella flexneri]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T160627 NZ_CP070593:4823495-4823806 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13698.52 Da        Isoelectric Point: 5.9505

>AT160627 WP_001285620.1 NZ_CP054913:4325771-4326148 [Shigella flexneri]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Download         Length: 378 bp

>AT160627 NZ_CP070593:4823807-4824097 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6M7

Antitoxin

Source ID Structure
PDB 2INW
AlphaFold DB A0A0E0Y522

References