Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2432346..2433141 Replicon chromosome
Accession NZ_CP054913
Organism Shigella flexneri strain FDAARGOS_691

Toxin (Protein)


Gene name yeeV Uniprot ID Q93EZ7
Locus tag FOB99_RS11925 Protein ID WP_000854917.1
Coordinates 2432767..2433141 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D2ACA1
Locus tag FOB99_RS11920 Protein ID WP_001280958.1
Coordinates 2432346..2432720 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB99_RS11895 2429461..2429619 + 159 WP_001323397.1 DUF905 family protein -
FOB99_RS11900 2429774..2430592 + 819 WP_001234651.1 DUF945 domain-containing protein -
FOB99_RS11905 2430934..2431407 + 474 WP_005011597.1 antirestriction protein -
FOB99_RS11910 2431423..2431899 + 477 WP_005011624.1 RadC family protein -
FOB99_RS11915 2431962..2432183 + 222 WP_001220314.1 DUF987 domain-containing protein -
FOB99_RS11920 2432346..2432720 + 375 WP_001280958.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
FOB99_RS11925 2432767..2433141 + 375 WP_000854917.1 TA system toxin CbtA family protein Toxin
FOB99_RS11930 2433138..2433629 + 492 WP_000976828.1 hypothetical protein -
FOB99_RS11935 2433641..2433838 + 198 WP_000839282.1 DUF957 domain-containing protein -
FOB99_RS11940 2433923..2434483 + 561 Protein_2308 DUF4942 domain-containing protein -
FOB99_RS11945 2434555..2435559 + 1005 WP_001179707.1 IS110-like element ISSfl8 family transposase -
FOB99_RS11950 2435841..2436122 + 282 Protein_2310 DUF4942 domain-containing protein -
FOB99_RS11960 2436594..2437532 + 939 Protein_2311 glyoxylate/hydroxypyruvate reductase GhrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2434555..2435559 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14145.22 Da        Isoelectric Point: 7.7761

>T160612 WP_000854917.1 NZ_CP054913:2432767-2433141 [Shigella flexneri]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T160612 NZ_CP070593:c1023624-1023406 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13793.62 Da        Isoelectric Point: 6.4651

>AT160612 WP_001280958.1 NZ_CP054913:2432346-2432720 [Shigella flexneri]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT160612 NZ_CP070593:c1024024-1023650 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q93EZ7

Antitoxin

Source ID Structure
AlphaFold DB A0A0K4PAV2

References