Detailed information of TA system TA160606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 554753..556101
• Replicon: chromosome
• Accession: NZ_CP054912
• Organism: Pantoea ananatis strain FDAARGOS_680

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: FOB88_RS05615
• Protein ID: WP_176017701.1
• Coordinates: 554992..556101 (+)
• Length: 370 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FOB88_RS05610
• Protein ID: WP_072138073.1
• Coordinates: 554753..554992 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB88_RS05595	550345..551733	+	1389	WP_014605798.1	MFS transporter	-
FOB88_RS05600	551756..554131	+	2376	WP_176017699.1	glycoside hydrolase family 3 C-terminal domain-containing protein	-
FOB88_RS05605	554195..554638	-	444	WP_176017700.1	GNAT family N-acetyltransferase	-
FOB88_RS05610	554753..554992	+	240	WP_072138073.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
FOB88_RS05615	554992..556101	+	1110	WP_176017701.1	HipA domain-containing protein	Toxin
FOB88_RS05620	556147..556563	-	417	WP_013025826.1	GFA family protein	-
FOB88_RS05625	557013..558881	-	1869	WP_176017702.1	low affinity potassium transporter Kup	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-63)

Sequences

Toxin

Protein

• Length: 370 a.a.
• Molecular weight: 40861.16 Da
• Isoelectric Point: 7.4732

>T160606 WP_176017701.1 NZ_CP054912:554992-556101 [Pantoea ananatis]
MAKLIAWINGERTGTLTLHHNGALHFQYDANWLQHPLARPLSLSLPLQRAAMHSEKVIHFFENLLFPSTLIEKDFYPTHP
IKSCEVFAALQKVGHTLPGALSLLPAKRQPVAPDQATHSVFSPANEAHQFDIDNAYLCLSLARELTIPVPDAVLVHENGI
RKLRTERYDRRWRHDGQHLYHLPEEDVCQALGVPLSGRDEQQGGPGIDDIMALLMGSSSAMKDREDMMRCLVFQWLTGIT
RVNAKHLSLLIARGGSFRLAPFKHLLSACPGLSLRTTDRQDFQLGMGVTGPEGKQHHIDAIGPAHFLALAGSVGFSQAAM
RQILAAFIPGLPAAIVKVRDSLPDDYPLTLADAVFSHAMMMLERVRQAT

Nucleotide

• Length: 1110 bp

>T160606 NZ_CP070593:282411-282761 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8745.96 Da
• Isoelectric Point: 8.7055

>AT160606 WP_072138073.1 NZ_CP054912:554753-554992 [Pantoea ananatis]
MIYSPRQLANQVRLLRQQNGWTQVELAKKVGLKQATLSHFENAPDTTSLATLFKILQALNLALSLHENSASSAPAEDAW

Nucleotide

• Length: 240 bp

>AT160606 NZ_CP070593:282166-282417 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA