Detailed information of TA system TA160596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 102860..103450
• Replicon: chromosome
• Accession: NZ_CP054912
• Organism: Pantoea ananatis strain FDAARGOS_680

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: FOB88_RS03345
• Protein ID: WP_176017524.1
• Coordinates: 103118..103450 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: FOB88_RS03340
• Protein ID: WP_014605554.1
• Coordinates: 102860..103117 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB88_RS03320	98121..98594	+	474	WP_176017522.1	pentapeptide repeat-containing protein	-
FOB88_RS03325	98870..100108	-	1239	WP_176017523.1	MFS transporter	-
FOB88_RS03330	100843..101805	+	963	WP_042678147.1	ferrochelatase	-
FOB88_RS03335	102307..102512	+	206	Protein_91	helix-turn-helix domain-containing protein	-
FOB88_RS03340	102860..103117	+	258	WP_014605554.1	antitoxin	Antitoxin
FOB88_RS03345	103118..103450	+	333	WP_176017524.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FOB88_RS03355	103711..104544	-	834	WP_013026205.1	DgsA anti-repressor MtfA	-
FOB88_RS03360	104650..105327	-	678	WP_176017525.1	hypothetical protein	-
FOB88_RS03365	105939..106643	+	705	WP_022623305.1	phosphohydrolase	-
FOB88_RS03370	106717..106971	+	255	WP_013026202.1	Arc family DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-83)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12002.75 Da
• Isoelectric Point: 5.8426

>T160596 WP_176017524.1 NZ_CP054912:103118-103450 [Pantoea ananatis]
MDRGEIWLVSLDPIAGHEQSGRRPVLIVSPASFNQFTRLPVVVPVTNGGNFARAAGFTVSLDGAETKTTGVIRCDQPRTL
DMEARNGKYLERIPEVIVNEVVARLEAILS

Nucleotide

• Length: 333 bp

>T160596 NZ_CP070586:1940657-1940759 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9253.73 Da
• Isoelectric Point: 4.5821

>AT160596 WP_014605554.1 NZ_CP054912:102860-103117 [Pantoea ananatis]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDSGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GKELL

Nucleotide

• Length: 258 bp

>AT160596 NZ_CP070586:c1940766-1940621 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT