Detailed information of TA system TA160594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 96822..98448
• Replicon: plasmid unnamed3
• Accession: NZ_CP054911
• Organism: Pantoea ananatis strain FDAARGOS_680

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: FOB88_RS02260
• Protein ID: WP_176017381.1
• Coordinates: 97123..98448 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FOB88_RS02255
• Protein ID: WP_176017380.1
• Coordinates: 96822..97118 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB88_RS02235	92236..92607	-	372	WP_176017376.1	hypothetical protein	-
FOB88_RS02245	94038..94487	+	450	WP_176017378.1	hypothetical protein	-
FOB88_RS02250	95581..96618	+	1038	WP_176017379.1	tyrosine-type recombinase/integrase	-
FOB88_RS02255	96822..97118	+	297	WP_176017380.1	helix-turn-helix transcriptional regulator	Antitoxin
FOB88_RS02260	97123..98448	+	1326	WP_176017381.1	type II toxin-antitoxin system HipA family toxin	Toxin
FOB88_RS02265	98608..98799	-	192	Protein_96	transposase	-
FOB88_RS02270	98915..100026	+	1112	WP_176017382.1	IS3 family transposase	-
FOB88_RS02275	100476..101465	-	990	WP_176017383.1	ParB/RepB/Spo0J family partition protein	-
FOB88_RS02280	101462..102670	-	1209	WP_104933885.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fliC	1..218767	218767

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-68)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50053.36 Da
• Isoelectric Point: 7.4625

>T160594 WP_176017381.1 NZ_CP054911:97123-98448 [Pantoea ananatis]
MNRRQQPLSIWMNGIRVGFWEKHRGEDSLRYLPEWCADEQGRSLSLSLPFTPGNQVWRGGVVRDYFDNLLPDSEGIRRRL
AMRYGAESLEPFDLLSELGRDCVGAIQLLRADEEPTGLYSVKYRLLSESEIATTLRNTTNAALPGRQDDLGDLRLSIAGA
QEKTALLWHDEHWCIPEAHTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSKLLENFGIPVASTHIAQFEEQKALVVERF
DRKWSANRQWLIRIPQEDMCQALGVSPLRKYQVDGGPGISQIMEILSHSDQAGLDRLRFFSAQIIFWLIAATDGHAKNFS
IVSGPQGSYRLTPLYDVLSAWPVIGGGNNQIALQKCKLAMAVRGSSNYYHIGRIQRRHWIKHGELTGLSKQQIEKMMEEI
TLRTPEVIERVSNLLPQAFPQVLAEKIFEGMRYQCRLLIGK

Nucleotide

• Length: 1326 bp

>T160594 NZ_CP070586:699793-700170 [Klebsiella pneumoniae]
ATGCAAACCCAACCACTATCCTCAACTCAGGAGGCTACACCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACACTCAACGACACTCCGTTCGGCAACGATGGCGTGATTCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAATTTTATCGTGGAGAAATACGATCTGGTACGAACCGACCGTCGCGGTTTT
AACGCAGAAACACAGTCGCCTCTTCTCAGCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGACTGATGACCCGAAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGGGAGGTGCAGCCATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11094.67 Da
• Isoelectric Point: 8.4697

>AT160594 WP_176017380.1 NZ_CP054911:96822-97118 [Pantoea ananatis]
MKNFYTVKTLSQLRPLLIAFRKEKGLTQKDISERLGVTQQTYARMEANPASAGIERLYRVLTLLGVEINLSSEASSSDVE
RMEETTSLSDSPARLEKW

Nucleotide

• Length: 297 bp

>AT160594 NZ_CP070586:699416-699742 [Klebsiella pneumoniae]
ATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTTTGGCGCACGGCTGGTACAGGCGGGCAACCGTCT
GCATTATCTCGCTGACCGGGCCAACATCACCGGACAGTTCAGCGACGCTGATTTACGGTATCTCGACCAGGCATTTCCAC
TGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCAATCCCTGCCATCAGCACTGTGTCACGCTCTATGCA
AAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGTATACCTGGCGATTTATCCGACACCAGCGGCAAC
CGCATAA