Detailed information of TA system TA160593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 63465..64018
• Replicon: plasmid unnamed3
• Accession: NZ_CP054911
• Organism: Pantoea ananatis strain FDAARGOS_680

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: FOB88_RS02105
• Protein ID: WP_176017357.1
• Coordinates: 63704..64018 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: FOB88_RS02100
• Protein ID: WP_176017356.1
• Coordinates: 63465..63701 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB88_RS02075	58474..59034	+	561	Protein_58	helix-turn-helix domain-containing protein	-
FOB88_RS02080	59392..60270	+	879	WP_176017472.1	protein RepA	-
FOB88_RS02085	60813..61933	+	1121	WP_176017355.1	IS3 family transposase	-
FOB88_RS02090	62049..62204	+	156	Protein_61	transposase	-
FOB88_RS02095	62321..63046	+	726	Protein_62	DUF4113 domain-containing protein	-
FOB88_RS02100	63465..63701	+	237	WP_176017356.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
FOB88_RS02105	63704..64018	+	315	WP_176017357.1	CcdB family protein	Toxin
FOB88_RS02110	64539..65102	-	564	WP_176017358.1	disulfide bond formation protein B	-
FOB88_RS02115	65483..65704	-	222	Protein_66	hypothetical protein	-
FOB88_RS02120	66210..67340	-	1131	WP_176017359.1	cyclopropane fatty acyl phospholipid synthase	-
FOB88_RS02125	67406..67669	-	264	WP_176017360.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fliC	1..218767	218767

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11668.68 Da
• Isoelectric Point: 7.9845

>T160593 WP_176017357.1 NZ_CP054911:63704-64018 [Pantoea ananatis]
MQFTVYSNTGKSAVYPLLLDVTSDIIEQLNRRIVIPLLPVKKYPSSTRPERLVPLIRLTDDKEYAVMTHEMASIPARALG
AVFCNASLYRTEIKAAIDFLLDGI

Nucleotide

• Length: 315 bp

>T160593 NZ_CP070586:391923-392270 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTCTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9025.04 Da
• Isoelectric Point: 4.6234

>AT160593 WP_176017356.1 NZ_CP054911:63465-63701 [Pantoea ananatis]
MAITQRVTQSVTMTVERDLLSRAREAGINLSATLTMALDAELRQYEAKKWQEENSEALEALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT160593 NZ_CP070586:392270-392569 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA