Detailed information of TA system TA160590

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5585836..5586459
• Replicon: chromosome
• Accession: NZ_CP054880
• Organism: Pseudomonas sp. 43A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: J3FUJ4
• Locus tag: HUW52_RS24450
• Protein ID: WP_007911472.1
• Coordinates: 5585836..5586015 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: J2R7W3
• Locus tag: HUW52_RS24455
• Protein ID: WP_007911473.1
• Coordinates: 5586052..5586459 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW52_RS24425	5581651..5583138	+	1488	WP_175555114.1	sensor domain-containing diguanylate cyclase	-
HUW52_RS24430	5583131..5583361	+	231	WP_038358305.1	cysteine-rich CWC family protein	-
HUW52_RS24435	5583395..5584087	+	693	WP_175555115.1	pseudouridine synthase	-
HUW52_RS24440	5584405..5585289	+	885	WP_175555116.1	alpha/beta hydrolase	-
HUW52_RS24450	5585836..5586015	+	180	WP_007911472.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUW52_RS24455	5586052..5586459	+	408	WP_007911473.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HUW52_RS24460	5586823..5588037	+	1215	WP_016982765.1	aldose 1-epimerase family protein	-
HUW52_RS24465	5588255..5588617	-	363	WP_016982766.1	hypothetical protein	-
HUW52_RS24470	5589182..5589925	+	744	WP_175555117.1	class I SAM-dependent methyltransferase	-
HUW52_RS24475	5590071..5590841	-	771	WP_175555118.1	trans-aconitate 2-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6632.90 Da
• Isoelectric Point: 11.1923

>T160590 WP_007911472.1 NZ_CP054880:5585836-5586015 [Pseudomonas sp. 43A]
VNSRFLIGQLVADGWYLVRIKGSHHHFKHPSKPGLVTVPHPKKDLLKKTAISILQQALL

Nucleotide

• Length: 180 bp

>T160590 NZ_CP070582:54857-55339 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14549.58 Da
• Isoelectric Point: 4.7324

>AT160590 WP_007911473.1 NZ_CP054880:5586052-5586459 [Pseudomonas sp. 43A]
MLYPIAISIGDDHHAWGVEVPDIPGCFSAGEDLDDAVAMAREAIEGHFEILADDDAPIPAASKLSLHVANPKYAGCAWAV
VDIDVLKYLGKAQKLNITLPGHLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQG

Nucleotide

• Length: 408 bp

>AT160590 NZ_CP070582:54603-54869 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: J3FUJ4

Antitoxin

• AlphaFold DB: J2R7W3