Detailed information of TA system TA160576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2120952..2121481
• Replicon: chromosome
• Accession: NZ_CP054876
• Organism: Staphylococcus aureus strain Dresden-275757

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HUW54_RS10550
• Protein ID: WP_000621175.1
• Coordinates: 2120952..2121314 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HUW54_RS10555
• Protein ID: WP_000948331.1
• Coordinates: 2121311..2121481 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW54_RS10530	2117930..2118700	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
HUW54_RS10535	2118675..2119154	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
HUW54_RS10540	2119156..2119482	-	327	WP_001052491.1	anti-sigma factor antagonist	-
HUW54_RS10545	2119601..2120602	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
HUW54_RS10550	2120952..2121314	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HUW54_RS10555	2121311..2121481	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HUW54_RS10560	2121566..2122714	-	1149	WP_001281154.1	alanine racemase	-
HUW54_RS10565	2122780..2123139	-	360	WP_000581197.1	holo-ACP synthase	-
HUW54_RS10570	2123143..2123634	-	492	WP_001205912.1	PH domain-containing protein	-
HUW54_RS10575	2123621..2125204	-	1584	WP_001294621.1	PH domain-containing protein	-
HUW54_RS10580	2125197..2125676	-	480	WP_001287079.1	hypothetical protein	-
HUW54_RS10585	2125885..2126445	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T160576 WP_000621175.1 NZ_CP054876:c2121314-2120952 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T160576 NZ_CP070578:84275-84625 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT160576 WP_000948331.1 NZ_CP054876:c2121481-2121311 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT160576 NZ_CP070578:84630-84932 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1