Detailed information of TA system TA160565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5974253..5974872
• Replicon: chromosome
• Accession: NZ_CP054874
• Organism: Pseudomonas protegens strain PGNL1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HUT20_RS27120
• Protein ID: WP_041752740.1
• Coordinates: 5974253..5974435 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2C9EK34
• Locus tag: HUT20_RS27125
• Protein ID: WP_015634980.1
• Coordinates: 5974471..5974872 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUT20_RS27105 (HUT20_27105)	5970018..5971805	-	1788	WP_015634985.1	1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein	-
HUT20_RS27110 (HUT20_27110)	5971783..5973021	-	1239	WP_015634984.1	aminotransferase class III-fold pyridoxal phosphate-dependent enzyme	-
HUT20_RS27115 (HUT20_27115)	5973042..5973560	-	519	WP_053150430.1	sugar O-acetyltransferase	-
HUT20_RS27120 (HUT20_27120)	5974253..5974435	+	183	WP_041752740.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUT20_RS27125 (HUT20_27125)	5974471..5974872	+	402	WP_015634980.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HUT20_RS27130 (HUT20_27130)	5975028..5976431	-	1404	WP_015634979.1	PLP-dependent aminotransferase family protein	-
HUT20_RS27135 (HUT20_27135)	5976519..5976965	+	447	WP_041752112.1	GNAT family N-acetyltransferase	-
HUT20_RS27140 (HUT20_27140)	5976962..5977582	+	621	WP_015634977.1	NAD(P)H-dependent oxidoreductase	-
HUT20_RS27145 (HUT20_27145)	5977611..5978042	+	432	WP_011060488.1	carboxymuconolactone decarboxylase family protein	-
HUT20_RS27150 (HUT20_27150)	5978276..5978830	-	555	WP_041752110.1	hypothetical protein	-
HUT20_RS27155 (HUT20_27155)	5979060..5979407	+	348	WP_015634975.1	metalloregulator ArsR/SmtB family transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6959.13 Da
• Isoelectric Point: 10.9595

>T160565 WP_041752740.1 NZ_CP054874:5974253-5974435 [Pseudomonas protegens]
VQSRQLIKELEATGWVLERVTESHHLFKHPYRPNTVPAPHPKKDLPRGTVRAIRKLAELI

Nucleotide

• Length: 183 bp

>T160565 NZ_CP070572:c114721-114338 [Proteus mirabilis]
ATGATATGGGTTAGCGCACAGGAAGTTATCGCTTTCCATGATCGTATATTACAACGTTTCCCAGGTGTGGCCGGTATGTC
CGATCCTGGCAGAGCGGAAGCTCTTATTTACCGGGTGCAAAACCGTAAACATTATGAAGGCATTACAGATGTGTTTGAAC
TGGCAGCAACTTATTGGGTCGCTATCGCTCGAGGGCACATATTTAATGACGGTAATAAACGTACCGCATTTTTTGTGACC
ATGACATTTCTCTATCGTAATGGCATCAGGATCCGCGATACTGGCAATATGCTGGAAAACTTAACAGTAGAAGCAGCTAC
AGGTGAGAAAACCGTCGAGCAGTTAGCAAAACATCTACAAAACTTGGTAGAGAAAACTAACTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14582.57 Da
• Isoelectric Point: 5.1666

>AT160565 WP_015634980.1 NZ_CP054874:5974471-5974872 [Pseudomonas protegens]
VQYPICIEWGDEHTATGIQIPDIPGAVTAGDSFESAYSAAIEIAHVMLEELARAGHAIPLPSPTRKHRANPDFEGMGWGM
LDIDIAPYLGKTEKVNVTLPGYVIQQIDRFVREHNIKSRSSFLADAALEKLGR

Nucleotide

• Length: 402 bp

>AT160565 NZ_CP070572:c114939-114718 [Proteus mirabilis]
ATGCGTACATATACCTCGACACAAGCCCGAGCCAATATTTCAGAAGTATTAGATATTGCGACCCACGGTGAACCTGTTGA
AATCACCCGCAGAGACGGTTGTTCAGCGGTTGTTATCAGTAAAACTGAATTTGAGTCATACCAGAATACTAAGCTAGATG
CAGAGTTTGATATGATGATGCAACGTCATGGACATACCGTAGAGGCACTGACAAATCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A2C9EK34