Detailed information of TA system TA160560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2072342..2072961
• Replicon: chromosome
• Accession: NZ_CP054873
• Organism: Pseudomonas protegens strain PGNR2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HUT21_RS09215
• Protein ID: WP_041752740.1
• Coordinates: 2072342..2072524 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2C9EK34
• Locus tag: HUT21_RS09220
• Protein ID: WP_015634980.1
• Coordinates: 2072560..2072961 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUT21_RS09200 (HUT21_09200)	2068107..2069894	-	1788	WP_015634985.1	1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein	-
HUT21_RS09205 (HUT21_09205)	2069872..2071110	-	1239	WP_015634984.1	aminotransferase class III-fold pyridoxal phosphate-dependent enzyme	-
HUT21_RS09210 (HUT21_09210)	2071131..2071649	-	519	WP_053150430.1	sugar O-acetyltransferase	-
HUT21_RS09215 (HUT21_09215)	2072342..2072524	+	183	WP_041752740.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUT21_RS09220 (HUT21_09220)	2072560..2072961	+	402	WP_015634980.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HUT21_RS09225 (HUT21_09225)	2073117..2074520	-	1404	WP_015634979.1	PLP-dependent aminotransferase family protein	-
HUT21_RS09230 (HUT21_09230)	2074608..2075054	+	447	WP_041752112.1	GNAT family N-acetyltransferase	-
HUT21_RS09235 (HUT21_09235)	2075051..2075671	+	621	WP_015634977.1	NAD(P)H-dependent oxidoreductase	-
HUT21_RS09240 (HUT21_09240)	2075700..2076131	+	432	WP_011060488.1	carboxymuconolactone decarboxylase family protein	-
HUT21_RS09245 (HUT21_09245)	2076365..2076919	-	555	WP_041752110.1	hypothetical protein	-
HUT21_RS09250 (HUT21_09250)	2077149..2077496	+	348	WP_015634975.1	metalloregulator ArsR/SmtB family transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6959.13 Da
• Isoelectric Point: 10.9595

>T160560 WP_041752740.1 NZ_CP054873:2072342-2072524 [Pseudomonas protegens]
VQSRQLIKELEATGWVLERVTESHHLFKHPYRPNTVPAPHPKKDLPRGTVRAIRKLAELI

Nucleotide

• Length: 183 bp

>T160560 NZ_CP070569:3878018-3878404 [Proteus mirabilis]
ATGGTTATTTATAAAACAAAGATGTTCAAAAATAGTTTCAAAAAAATAACGCTTACGAATGCAGAACTAATCGCTATCGC
TACCGAAGTCCTTGCAGGACAATTTGAGGCAGATCTAGGTGGTGGTGTTATCAAGAAACGTGCCTGTATCCAAGGAAAAG
GTAAAAGTAGCGGAATTAGAACAATTATATTTTATAAGCAAGGGAATAATTTATTCTTTGCTGATGGTTGGAAAAAATCG
AGTTTAAGCTCTAAAAAAACCAAAGAGATCACAGATGATGAACTTGAGTCCTATAAAGATCTAGCAAAAGATCTCTTCAA
TGCAAATCAAAATAAAATAGATAAAATGATTGCACTAGGCCTTTTAACAGAGGTTAGATATGACTGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14582.57 Da
• Isoelectric Point: 5.1666

>AT160560 WP_015634980.1 NZ_CP054873:2072560-2072961 [Pseudomonas protegens]
VQYPICIEWGDEHTATGIQIPDIPGAVTAGDSFESAYSAAIEIAHVMLEELARAGHAIPLPSPTRKHRANPDFEGMGWGM
LDIDIAPYLGKTEKVNVTLPGYVIQQIDRFVREHNIKSRSSFLADAALEKLGR

Nucleotide

• Length: 402 bp

>AT160560 NZ_CP070569:3878397-3878717 [Proteus mirabilis]
ATGACTGATAAAATGAAAGAAATGCTAAAATTTGCACAAGAAATGGAAGATGCAGGAGTCACTGATGGCAGTACAACTAA
ACGTATTGCCGCCAGAATAAAAGCGAAAGAATTACAGGCTAAGTATAAACATCCTACCCCTATGACAGGTGAAGATATAA
AAAGGATCCGTGAGCAAAATAATATCTCTCAATCTTCCCTTGCTTTGATATTGAATATGACTAAAGAAAGCGTTTCTAAG
TGGGAAAGAAACGAAATTAAGCCCAATGGTGCAGCACTACGACTTCTGCATTTAATAGATAAAAAAATATTATTCATCTA
G

Structures

Antitoxin

• AlphaFold DB: A0A2C9EK34