Detailed information of TA system TA160553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2653920..2654509
• Replicon: chromosome
• Accession: NZ_CP054871
• Organism: Pseudomonas protegens strain PPRAR07

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7V8EXE5
• Locus tag: HUT23_RS12230
• Protein ID: WP_019094202.1
• Coordinates: 2654258..2654509 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUT23_RS12225
• Protein ID: WP_072451388.1
• Coordinates: 2653920..2654261 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUT23_RS12200 (HUT23_12215)	2649265..2650221	-	957	WP_210456996.1	nucleoside-specific channel-forming protein Tsx	-
HUT23_RS12205 (HUT23_12220)	2650483..2650884	+	402	WP_232642573.1	nuclear transport factor 2 family protein	-
HUT23_RS12210 (HUT23_12225)	2650928..2651962	+	1035	WP_015635068.1	selenide, water dikinase SelD	-
HUT23_RS12215 (HUT23_12230)	2651962..2653065	+	1104	WP_210456994.1	tRNA 2-selenouridine(34) synthase MnmH	-
HUT23_RS12220 (HUT23_12235)	2653118..2653459	-	342	WP_053157594.1	DUF3077 domain-containing protein	-
HUT23_RS12225 (HUT23_12240)	2653920..2654261	-	342	WP_072451388.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HUT23_RS12230 (HUT23_12245)	2654258..2654509	-	252	WP_019094202.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUT23_RS12235 (HUT23_12250)	2654598..2655653	-	1056	WP_210456992.1	permease	-
HUT23_RS12240 (HUT23_12255)	2656188..2656964	-	777	WP_011060579.1	IclR family transcriptional regulator	-
HUT23_RS12245 (HUT23_12260)	2657136..2657906	+	771	WP_183000119.1	ABC transporter substrate-binding protein	-
HUT23_RS12250 (HUT23_12265)	2657967..2658623	+	657	WP_011060581.1	amino acid ABC transporter permease	-
HUT23_RS12255 (HUT23_12270)	2658620..2659366	+	747	WP_011060582.1	amino acid ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2653920..2663972	10052

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9239.70 Da
• Isoelectric Point: 10.4707

>T160553 WP_019094202.1 NZ_CP054871:c2654509-2654258 [Pseudomonas protegens]
VSKNDKLLAKLLNDRGTFSWQELVTLLKRLGYTPLEGSGSRVKFDNGDPGAMINLHKPHPGNELKAYIRRQVIEQLKSGG
LIP

Nucleotide

• Length: 252 bp

>T160553 NZ_CP070568:4488764-4489174 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12438.82 Da
• Isoelectric Point: 4.8281

>AT160553 WP_072451388.1 NZ_CP054871:c2654261-2653920 [Pseudomonas protegens]
VNNLLQHKGYYGSIEVSTEDNCLFGKLQFIRALVSYEGQTVAELEAAFKEAVDDYLDTCEQSGTAPEIPCKGSFNVRVGH
DLHLAAAVAATRQNVSLNDLTRQALNEYLQHHG

Nucleotide

• Length: 342 bp

>AT160553 NZ_CP070568:4488517-4488783 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7V8EXE5