Detailed information of TA system TA160549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3177404..3178017
• Replicon: chromosome
• Accession: NZ_CP054868
• Organism: Pseudomonas chlororaphis strain PCLAR04

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A285EYZ4
• Locus tag: HUT26_RS14455
• Protein ID: WP_009041685.1
• Coordinates: 3177835..3178017 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUT26_RS14450
• Protein ID: WP_025808441.1
• Coordinates: 3177404..3177805 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUT26_RS14425 (HUT26_14430)	3172922..3173761	+	840	WP_063427785.1	taurine ABC transporter permease TauC	-
HUT26_RS14430 (HUT26_14435)	3173999..3174838	+	840	WP_106696248.1	taurine dioxygenase	-
HUT26_RS14435 (HUT26_14440)	3174978..3175223	+	246	WP_009046453.1	plasmid stabilization protein	-
HUT26_RS14440 (HUT26_14445)	3175220..3175648	+	429	WP_009046452.1	type II toxin-antitoxin system VapC family toxin	-
HUT26_RS14445 (HUT26_14450)	3175778..3176815	-	1038	WP_063427783.1	L-glyceraldehyde 3-phosphate reductase	-
HUT26_RS14450 (HUT26_14455)	3177404..3177805	-	402	WP_025808441.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HUT26_RS14455 (HUT26_14460)	3177835..3178017	-	183	WP_009041685.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUT26_RS14460 (HUT26_14465)	3178142..3179032	-	891	WP_025808442.1	LysR family transcriptional regulator	-
HUT26_RS14465 (HUT26_14470)	3179162..3179902	+	741	WP_063427782.1	SDR family oxidoreductase	-
HUT26_RS14470 (HUT26_14475)	3179959..3180438	+	480	WP_063427781.1	DoxX family protein	-
HUT26_RS14475 (HUT26_14480)	3180491..3180748	+	258	WP_063427780.1	hypothetical protein	-
HUT26_RS14480 (HUT26_14485)	3180871..3182835	-	1965	WP_164485791.1	choline transporter BetT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.08 Da
• Isoelectric Point: 10.4588

>T160549 WP_009041685.1 NZ_CP054868:c3178017-3177835 [Pseudomonas chlororaphis]
MRSRELIRMIEEDGWYLIAVKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Nucleotide

• Length: 183 bp

>T160549 NZ_CP070568:c3034165-3033632 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14568.38 Da
• Isoelectric Point: 4.9036

>AT160549 WP_025808441.1 NZ_CP054868:c3177805-3177404 [Pseudomonas chlororaphis]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVV
DFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT160549 NZ_CP070568:c3034442-3034176 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A285EYZ4