Detailed information of TA system TA160548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 3174978..3175648
• Replicon: chromosome
• Accession: NZ_CP054868
• Organism: Pseudomonas chlororaphis strain PCLAR04

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUT26_RS14440
• Protein ID: WP_009046452.1
• Coordinates: 3175220..3175648 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3G7DZE9
• Locus tag: HUT26_RS14435
• Protein ID: WP_009046453.1
• Coordinates: 3174978..3175223 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUT26_RS14415 (HUT26_14420)	3171044..3172021	+	978	WP_025808435.1	taurine ABC transporter substrate-binding protein	-
HUT26_RS14420 (HUT26_14425)	3172131..3172925	+	795	WP_063427786.1	taurine ABC transporter ATP-binding subunit	-
HUT26_RS14425 (HUT26_14430)	3172922..3173761	+	840	WP_063427785.1	taurine ABC transporter permease TauC	-
HUT26_RS14430 (HUT26_14435)	3173999..3174838	+	840	WP_106696248.1	taurine dioxygenase	-
HUT26_RS14435 (HUT26_14440)	3174978..3175223	+	246	WP_009046453.1	plasmid stabilization protein	Antitoxin
HUT26_RS14440 (HUT26_14445)	3175220..3175648	+	429	WP_009046452.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUT26_RS14445 (HUT26_14450)	3175778..3176815	-	1038	WP_063427783.1	L-glyceraldehyde 3-phosphate reductase	-
HUT26_RS14450 (HUT26_14455)	3177404..3177805	-	402	WP_025808441.1	type II toxin-antitoxin system HicB family antitoxin	-
HUT26_RS14455 (HUT26_14460)	3177835..3178017	-	183	WP_009041685.1	type II toxin-antitoxin system HicA family toxin	-
HUT26_RS14460 (HUT26_14465)	3178142..3179032	-	891	WP_025808442.1	LysR family transcriptional regulator	-
HUT26_RS14465 (HUT26_14470)	3179162..3179902	+	741	WP_063427782.1	SDR family oxidoreductase	-
HUT26_RS14470 (HUT26_14475)	3179959..3180438	+	480	WP_063427781.1	DoxX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15867.39 Da
• Isoelectric Point: 5.4927

>T160548 WP_009046452.1 NZ_CP054868:3175220-3175648 [Pseudomonas chlororaphis]
MIVLDTNVLSELMRPQPDTAVLLWIEAQPVEDLYISAMTMAEILHGIARLPHGKRKQTLHASALAMFEEDFIDRIVPFGT
QAALYYAHLVSHRERSGQPINLVDAQIAAICRVHDAAIATRNTKDFTDTGLIVINPWLPLEV

Nucleotide

• Length: 429 bp

>T160548 NZ_CP070568:2437559-2437777 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9103.27 Da
• Isoelectric Point: 6.2314

>AT160548 WP_009046453.1 NZ_CP054868:3174978-3175223 [Pseudomonas chlororaphis]
MASLTIRNLDDHIKERLRIEAAHNGHSMEEEARLILRRALSRTADSQGLGSRIRARFAETGGVELELPERTEPARLVDLG
E

Nucleotide

• Length: 246 bp

>AT160548 NZ_CP070568:2437158-2437532 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A3G7DZE9