Detailed information of TA system TA160544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5875232..5875821
• Replicon: chromosome
• Accession: NZ_CP054867
• Organism: Pseudomonas chlororaphis strain PCLRT02

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: HUT27_RS26235
• Protein ID: WP_124355458.1
• Coordinates: 5875522..5875821 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HUT27_RS26230
• Protein ID: WP_124355457.1
• Coordinates: 5875232..5875525 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUT27_RS26210 (HUT27_26210)	5870872..5872035	-	1164	WP_124315432.1	acyltransferase	-
HUT27_RS26215 (HUT27_26215)	5872230..5873420	-	1191	WP_124355455.1	serine hydrolase domain-containing protein	-
HUT27_RS26220 (HUT27_26220)	5873547..5874434	+	888	WP_009051074.1	LysR family transcriptional regulator	-
HUT27_RS26225 (HUT27_26225)	5874707..5875099	+	393	WP_124355456.1	hypothetical protein	-
HUT27_RS26230 (HUT27_26230)	5875232..5875525	-	294	WP_124355457.1	putative addiction module antidote protein	Antitoxin
HUT27_RS26235 (HUT27_26235)	5875522..5875821	-	300	WP_124355458.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUT27_RS26240 (HUT27_26240)	5875993..5876442	+	450	WP_124355459.1	DUF3828 domain-containing protein	-
HUT27_RS26245 (HUT27_26245)	5876467..5877477	-	1011	WP_177477863.1	NAD(P)-dependent alcohol dehydrogenase	-
HUT27_RS26250 (HUT27_26250)	5877535..5877834	-	300	WP_009051080.1	helix-turn-helix transcriptional regulator	-
HUT27_RS26255 (HUT27_26255)	5877898..5878989	-	1092	WP_041987461.1	copper-containing nitrite reductase	-
HUT27_RS26260 (HUT27_26260)	5879215..5879457	+	243	WP_007924102.1	exodeoxyribonuclease VII small subunit	-
HUT27_RS26265 (HUT27_26265)	5879454..5880341	+	888	WP_009051083.1	(2E,6E)-farnesyl diphosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-89)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11264.11 Da
• Isoelectric Point: 10.1871

>T160544 WP_124355458.1 NZ_CP054867:c5875821-5875522 [Pseudomonas chlororaphis]
MIEIKQIATFMEWENRLKDPRARALIAARIFRLANGLAGDVSPVGEGVSELRIHYGPGYRVYFQPCGLQLVILLCGGDKS
RQSRDIELAKYLARRWSRS

Nucleotide

• Length: 300 bp

>T160544 NZ_CP070568:159992-160324 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10426.93 Da
• Isoelectric Point: 5.6642

>AT160544 WP_124355457.1 NZ_CP054867:c5875525-5875232 [Pseudomonas chlororaphis]
MNEPLFDYDPATALRDPQAIALFMADAFETGDAGYIAKALGVVARAKGMTELAHETGLSREQLYRSFSDKGNPTLKTLLA
VMRALGLELTARVHGAS

Nucleotide

• Length: 294 bp

>AT160544 NZ_CP070568:159734-159991 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA