Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 2338000..2338585 Replicon chromosome
Accession NZ_CP054867
Organism Pseudomonas chlororaphis strain PCLRT02

Toxin (Protein)


Gene name PumA Uniprot ID A0A7S5HMS4
Locus tag HUT27_RS10550 Protein ID WP_016705075.1
Coordinates 2338292..2338585 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A8A9ZIA4
Locus tag HUT27_RS10545 Protein ID WP_009048177.1
Coordinates 2338000..2338290 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUT27_RS10535 (HUT27_10535) 2335512..2336894 + 1383 WP_009048175.1 efflux transporter outer membrane subunit -
HUT27_RS10540 (HUT27_10540) 2337013..2337600 + 588 WP_028682910.1 hypothetical protein -
HUT27_RS10545 (HUT27_10545) 2338000..2338290 - 291 WP_009048177.1 putative addiction module antidote protein Antitoxin
HUT27_RS10550 (HUT27_10550) 2338292..2338585 - 294 WP_016705075.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUT27_RS10555 (HUT27_10555) 2338820..2340214 - 1395 WP_041988399.1 VOC family protein -
HUT27_RS10560 (HUT27_10560) 2340479..2341603 + 1125 WP_124313190.1 diguanylate cyclase -
HUT27_RS10565 (HUT27_10565) 2341697..2342941 + 1245 WP_041988393.1 M20/M25/M40 family metallo-hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-97)

Antitoxin

Uncharacterized protein HI1420

(9-91)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10699.27 Da        Isoelectric Point: 10.0959

>T160542 WP_016705075.1 NZ_CP054867:c2338585-2338292 [Pseudomonas chlororaphis]
MDYEILQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNTGAGYRLYFTVRGNTLIVLLLGGDK
SSQPADICQARSLAKEF

Download         Length: 294 bp

>T160542 NZ_CP070565:45510-45926 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10279.84 Da        Isoelectric Point: 8.0096

>AT160542 WP_009048177.1 NZ_CP054867:c2338290-2338000 [Pseudomonas chlororaphis]
MSDTLVPFDIATLLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKTTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVPRG

Download         Length: 291 bp

>AT160542 NZ_CP070565:45283-45513 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7S5HMS4

Antitoxin

Source ID Structure
AlphaFold DB A0A8A9ZIA4

References