Detailed information of TA system TA160541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2314070..2314689
• Replicon: chromosome
• Accession: NZ_CP054867
• Organism: Pseudomonas chlororaphis strain PCLRT02

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3G7DMG3
• Locus tag: HUT27_RS10445
• Protein ID: WP_009048155.1
• Coordinates: 2314507..2314689 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUT27_RS10440
• Protein ID: WP_124313176.1
• Coordinates: 2314070..2314471 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUT27_RS10425 (HUT27_10425)	2310129..2312018	+	1890	WP_210532792.1	PAS domain S-box protein	-
HUT27_RS10430 (HUT27_10430)	2312015..2312650	+	636	WP_053260452.1	response regulator transcription factor	-
HUT27_RS10435 (HUT27_10435)	2312792..2313508	+	717	WP_246881602.1	hypothetical protein	-
HUT27_RS10440 (HUT27_10440)	2314070..2314471	-	402	WP_124313176.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HUT27_RS10445 (HUT27_10445)	2314507..2314689	-	183	WP_009048155.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUT27_RS10450 (HUT27_10450)	2315053..2315334	-	282	WP_210532794.1	hypothetical protein	-
HUT27_RS10455 (HUT27_10455)	2315735..2316091	-	357	WP_210532795.1	hypothetical protein	-
HUT27_RS10460 (HUT27_10460)	2316076..2316273	-	198	Protein_2059	hypothetical protein	-
HUT27_RS10465 (HUT27_10465)	2316405..2316686	-	282	WP_210532796.1	hypothetical protein	-
HUT27_RS10470 (HUT27_10470)	2317322..2317828	+	507	WP_045884260.1	hypothetical protein	-
HUT27_RS10475 (HUT27_10475)	2318222..2318860	+	639	WP_210532797.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6799.98 Da
• Isoelectric Point: 10.9678

>T160541 WP_009048155.1 NZ_CP054867:c2314689-2314507 [Pseudomonas chlororaphis]
VQSRQLIKELEAAGWVLERVTGSHHLFKHPYRPETVPVPHPKKDLPRGTVRAIQKLAGLI

Nucleotide

• Length: 183 bp

>T160541 NZ_CP070564:5227425-5227808 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14598.58 Da
• Isoelectric Point: 4.6034

>AT160541 WP_124313176.1 NZ_CP054867:c2314471-2314070 [Pseudomonas chlororaphis]
VQYPICIEWGDERTATGIQIPDIPGAVTAGDTFEAAYSAAIEIAHVMLEELAREGQAIPLPSPTGTHRANPDFEGMGWGM
LDIDITPYLGKTEKVNVTLPGYVIQRIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT160541 NZ_CP070564:5227183-5227425 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A3G7DMG3