Detailed information of TA system TA160534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 313515..314185
• Replicon: chromosome
• Accession: NZ_CP054865
• Organism: Pseudomonas chlororaphis strain PCLSL01

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUT29_RS01370
• Protein ID: WP_009046452.1
• Coordinates: 313515..313943 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3G7DZE9
• Locus tag: HUT29_RS01375
• Protein ID: WP_009046453.1
• Coordinates: 313940..314185 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUT29_RS01340 (HUT29_01340)	308725..309204	-	480	WP_063427781.1	DoxX family protein	-
HUT29_RS01345 (HUT29_01345)	309261..310001	-	741	WP_063427782.1	SDR family oxidoreductase	-
HUT29_RS01350 (HUT29_01350)	310131..311021	+	891	WP_025808442.1	LysR family transcriptional regulator	-
HUT29_RS01355 (HUT29_01355)	311146..311328	+	183	WP_009041685.1	type II toxin-antitoxin system HicA family toxin	-
HUT29_RS01360 (HUT29_01360)	311358..311759	+	402	WP_025808441.1	type II toxin-antitoxin system HicB family antitoxin	-
HUT29_RS01365 (HUT29_01365)	312348..313385	+	1038	WP_063427783.1	L-glyceraldehyde 3-phosphate reductase	-
HUT29_RS01370 (HUT29_01370)	313515..313943	-	429	WP_009046452.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUT29_RS01375 (HUT29_01375)	313940..314185	-	246	WP_009046453.1	plasmid stabilization protein	Antitoxin
HUT29_RS01380 (HUT29_01380)	314325..315164	-	840	WP_106696248.1	taurine dioxygenase	-
HUT29_RS01385 (HUT29_01385)	315402..316241	-	840	WP_063427785.1	taurine ABC transporter permease TauC	-
HUT29_RS01390 (HUT29_01390)	316238..317032	-	795	WP_063427786.1	taurine ABC transporter ATP-binding subunit	-
HUT29_RS01395 (HUT29_01395)	317142..318119	-	978	WP_025808435.1	taurine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15867.39 Da
• Isoelectric Point: 5.4927

>T160534 WP_009046452.1 NZ_CP054865:c313943-313515 [Pseudomonas chlororaphis]
MIVLDTNVLSELMRPQPDTAVLLWIEAQPVEDLYISAMTMAEILHGIARLPHGKRKQTLHASALAMFEEDFIDRIVPFGT
QAALYYAHLVSHRERSGQPINLVDAQIAAICRVHDAAIATRNTKDFTDTGLIVINPWLPLEV

Nucleotide

• Length: 429 bp

>T160534 NZ_CP070564:c1085652-1085434 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9103.27 Da
• Isoelectric Point: 6.2314

>AT160534 WP_009046453.1 NZ_CP054865:c314185-313940 [Pseudomonas chlororaphis]
MASLTIRNLDDHIKERLRIEAAHNGHSMEEEARLILRRALSRTADSQGLGSRIRARFAETGGVELELPERTEPARLVDLG
E

Nucleotide

• Length: 246 bp

>AT160534 NZ_CP070564:c1086053-1085679 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A3G7DZE9