Detailed information of TA system TA160533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 311146..311759
• Replicon: chromosome
• Accession: NZ_CP054865
• Organism: Pseudomonas chlororaphis strain PCLSL01

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A285EYZ4
• Locus tag: HUT29_RS01355
• Protein ID: WP_009041685.1
• Coordinates: 311146..311328 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUT29_RS01360
• Protein ID: WP_025808441.1
• Coordinates: 311358..311759 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUT29_RS01330 (HUT29_01330)	306328..308292	+	1965	WP_164485791.1	choline transporter BetT	-
HUT29_RS01335 (HUT29_01335)	308415..308672	-	258	WP_063427780.1	hypothetical protein	-
HUT29_RS01340 (HUT29_01340)	308725..309204	-	480	WP_063427781.1	DoxX family protein	-
HUT29_RS01345 (HUT29_01345)	309261..310001	-	741	WP_063427782.1	SDR family oxidoreductase	-
HUT29_RS01350 (HUT29_01350)	310131..311021	+	891	WP_025808442.1	LysR family transcriptional regulator	-
HUT29_RS01355 (HUT29_01355)	311146..311328	+	183	WP_009041685.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUT29_RS01360 (HUT29_01360)	311358..311759	+	402	WP_025808441.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HUT29_RS01365 (HUT29_01365)	312348..313385	+	1038	WP_063427783.1	L-glyceraldehyde 3-phosphate reductase	-
HUT29_RS01370 (HUT29_01370)	313515..313943	-	429	WP_009046452.1	type II toxin-antitoxin system VapC family toxin	-
HUT29_RS01375 (HUT29_01375)	313940..314185	-	246	WP_009046453.1	plasmid stabilization protein	-
HUT29_RS01380 (HUT29_01380)	314325..315164	-	840	WP_106696248.1	taurine dioxygenase	-
HUT29_RS01385 (HUT29_01385)	315402..316241	-	840	WP_063427785.1	taurine ABC transporter permease TauC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.08 Da
• Isoelectric Point: 10.4588

>T160533 WP_009041685.1 NZ_CP054865:311146-311328 [Pseudomonas chlororaphis]
MRSRELIRMIEEDGWYLIAVKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Nucleotide

• Length: 183 bp

>T160533 NZ_CP070564:472844-473377 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14568.38 Da
• Isoelectric Point: 4.9036

>AT160533 WP_025808441.1 NZ_CP054865:311358-311759 [Pseudomonas chlororaphis]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVV
DFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT160533 NZ_CP070564:472567-472833 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A285EYZ4