Detailed information of TA system TA160529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 1459399..1460069
• Replicon: chromosome
• Accession: NZ_CP054861
• Organism: Martelella sp. NC20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HQ843_RS06990
• Protein ID: WP_180899192.1
• Coordinates: 1459668..1460069 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HQ843_RS06985
• Protein ID: WP_180899193.1
• Coordinates: 1459399..1459671 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQ843_RS06980	1454616..1459340	+	4725	WP_180899194.1	glutamate synthase large subunit	-
HQ843_RS06985	1459399..1459671	+	273	WP_180899193.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HQ843_RS06990	1459668..1460069	+	402	WP_180899192.1	type II toxin-antitoxin system VapC family toxin	Toxin
HQ843_RS06995	1460115..1460438	+	324	WP_180899191.1	heavy metal-binding domain-containing protein	-
HQ843_RS07000	1460452..1461906	+	1455	WP_180899190.1	glutamate synthase subunit beta	-
HQ843_RS07005	1462061..1462948	+	888	WP_180899189.1	UTP--glucose-1-phosphate uridylyltransferase GalU	-
HQ843_RS07010	1463340..1464332	+	993	WP_180899188.1	KpsF/GutQ family sugar-phosphate isomerase	-
HQ843_RS07015	1464402..1464905	-	504	WP_180899187.1	disulfide bond formation protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-123)

Antitoxin

• SpoVT-AbrB domain (14-52)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15139.50 Da
• Isoelectric Point: 5.8050

>T160529 WP_180899192.1 NZ_CP054861:1459668-1460069 [Martelella sp. NC20]
VSYLFDTNAISDMIRRPAGKIAQRLVGLEKRDLKTSLIVAAELRYGVVKRGSKELSELMERVLSQFEILPWEPPADIRYA
ELRNHLRQIGQPIGDMDMLIAAHALAIDAVVVTANEREFERVPGLKVENWERD

Nucleotide

• Length: 402 bp

>T160529 NZ_CP070560:61416-61832 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10484.09 Da
• Isoelectric Point: 4.6768

>AT160529 WP_180899193.1 NZ_CP054861:1459399-1459671 [Martelella sp. NC20]
MSNRITKEKKSRIFMNGRSRAVRIPSEFELEGDEVIIRQEESGLITIEPVKKKMTPRELIEWLRSKAPLGEELPEIEDLP
PREVDLDLPE

Nucleotide

• Length: 273 bp

>AT160529 NZ_CP070560:61189-61419 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA