Detailed information of TA system TA160523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4922900..4924473
• Replicon: chromosome
• Accession: NZ_CP054854
• Organism: Aeromonas dhakensis strain 1706-28330

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HUI95_RS22715
• Protein ID: WP_206866181.1
• Coordinates: 4922900..4924219 (-)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A3Q8DTG9
• Locus tag: HUI95_RS22720
• Protein ID: WP_042047685.1
• Coordinates: 4924219..4924473 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUI95_RS22700	4918791..4919555	+	765	WP_139431889.1	ATP-binding cassette domain-containing protein	-
HUI95_RS22705	4919574..4920500	+	927	WP_206866179.1	ABC transporter substrate-binding protein	-
HUI95_RS22710	4920497..4922491	+	1995	WP_206866180.1	Fe(3+)-hydroxamate ABC transporter permease FhuB	-
HUI95_RS22715	4922900..4924219	-	1320	WP_206866181.1	HipA domain-containing protein	Toxin
HUI95_RS22720	4924219..4924473	-	255	WP_042047685.1	helix-turn-helix domain-containing protein	Antitoxin
HUI95_RS22725	4924877..4925917	+	1041	WP_206865458.1	IS481 family transposase	-
HUI95_RS22730	4925967..4926500	-	534	Protein_4383	phage integrase SAM-like domain-containing protein	-
HUI95_RS22735	4926663..4928024	-	1362	WP_017410486.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4924877..4925917	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49545.62 Da
• Isoelectric Point: 6.9649

>T160523 WP_206866181.1 NZ_CP054854:c4924219-4922900 [Aeromonas dhakensis]
MANLAVYMNGYRVGTFTKTTSGTHQFQYRESWLAQPGSRPISLSMPLRHQPYRGDEAYNFFDNLLPDNMEVRNRVVARYQ
AASTQPFDLLSCIGQDSVGALQLVTEGHDVPDVRRIDYKALSDDELEQILTSYKSGIPLGMVREQEEFRISIAGAQEKTA
LLYLDGRWYLPLNATPTTHIIKLPIGKIESHSYSIDLSQSVENEYLCTLIAKEFGLPVPHCFMMQVGQVKALAVERFDRR
YAADRSWIMRLPQEDFCQVLNVPSARKYESHGGPGITDIMKTLLGSATPEQDRYLFMKSQVLFWLLAATDGHAKNFSVFI
EPEGRFRLTPFYDILSVYPVFGGRGLNRRDAKLAMGLASSKGKKYAIEQIFPRHFFQTAKAVGFERSAMEEILTELANSV
DDVIERVTQQLPAGFPDAISSTILEGLKARSARLIKGWD

Nucleotide

• Length: 1320 bp

>T160523 NZ_CP070549:c4774155-4773937 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGTCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9293.62 Da
• Isoelectric Point: 7.9834

>AT160523 WP_042047685.1 NZ_CP054854:c4924473-4924219 [Aeromonas dhakensis]
MKVTSAKQLSACIKDARLSQKLSQGKVAEKVGIRQDTVSSFELNPESTKLETLFKILAALELSLDIKPRNESSDSPASGW
KEEW

Nucleotide

• Length: 255 bp

>AT160523 NZ_CP070549:c4774557-4774183 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A3Q8DTG9