Detailed information of TA system TA160521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 5064180..5065438
• Replicon: chromosome
• Accession: NZ_CP054845
• Organism: Pseudomonas aeruginosa strain SE5429

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: HUS13_RS23775
• Protein ID: WP_003086331.1
• Coordinates: 5064180..5064935 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HUS13_RS23780
• Protein ID: WP_003086329.1
• Coordinates: 5065070..5065438 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS13_RS23765	5059351..5061894	+	2544	WP_003134033.1	acyl-homoserine-lactone acylase QuiP	-
HUS13_RS23770	5062049..5063410	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
HUS13_RS23775	5064180..5064935	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
HUS13_RS23780	5065070..5065438	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HUS13_RS23785	5065511..5066797	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
HUS13_RS23790	5066853..5068442	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
HUS13_RS23795	5068521..5068997	+	477	WP_003086322.1	hypothetical protein	-
HUS13_RS23800	5069000..5069275	-	276	WP_023098713.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T160521 WP_003086331.1 NZ_CP054845:c5064935-5064180 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T160521 NZ_CP070549:c3940409-3940029 [Citrobacter freundii]
ATGCAAACCTTATCCGCCATCCCGAAGCGGAAGGCACCGTCACGCCCGACGCCCGTTGAAATCTGGCAGCAACTTCTTAC
GTATCTGTTAAAAAGACATTACGGCCTTTCGCTTAGCGACACGCAATTTTCCGATGAGGAGATTATTACGCAATATATTG
ACGCCGGTATCTCACTGTCAGATGCACTCAATTTTCTCGTTGAAAAATCTGAACTGGTGCGGATTGATCGTCCCGGTTTC
TCCATTAAGCATCAGTCCCCTTTTATCGGGGTGATTGATATTCTCCGGGCCAGACGGGCAACGGGTTTGATGCAACGTAA
CGGGTATAAAAGGATCACTCTTCTCATCGCTGGAAATGCAGCTCAGGAGCAACATTCATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT160521 WP_003086329.1 NZ_CP054845:c5065438-5065070 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT160521 NZ_CP070549:c3940832-3940467 [Citrobacter freundii]
TTGTCGAATCCAATACCTCCAGTAAACCATGATGTCTCTGAGCCCTGGTGGGGGCTGAAACCGGGCATCACTCCGTGTTT
TGGCGCACGACTGGTACAGGAAGGCAACCGCCTGCATTATCTGGCTGATCGGGCCAGTATCGCCGGTGTGTTCAGCGATG
CGGATTTACGCCATCTCGATCAAGCATTTCCGGTACTGCTCAAACAGCTGGAATTAATGCTGGTTTCCGGTGAGCTCAAT
CCCTGCCATCAGCACTGTGTCACGTTATATGCAAAAGGGCTGACCTGTGAAGCAGATTCCCTCGGATCACATGGCTACAT
TTATACAGCAATTTATCCGACACCAGACGACAGCATAACCCGGTAA

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7