Detailed information of TA system TA160520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 4038490..4039711
• Replicon: chromosome
• Accession: NZ_CP054845
• Organism: Pseudomonas aeruginosa strain SE5429

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: V6AG16
• Locus tag: HUS13_RS18835
• Protein ID: WP_003088107.1
• Coordinates: 4039133..4039711 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUS13_RS18830
• Protein ID: WP_003088112.1
• Coordinates: 4038490..4039044 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS13_RS18805	4034058..4034609	+	552	WP_023098678.1	XRE family transcriptional regulator	-
HUS13_RS18810	4034606..4035004	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HUS13_RS18815	4035017..4035340	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
HUS13_RS18820	4035671..4036132	+	462	WP_003088117.1	(2Fe-2S)-binding protein	-
HUS13_RS18825	4036137..4038332	+	2196	WP_003088114.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HUS13_RS18830	4038490..4039044	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
HUS13_RS18835	4039133..4039711	+	579	WP_003088107.1	HD domain-containing protein	Toxin
HUS13_RS18840	4039820..4041007	-	1188	WP_003088106.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HUS13_RS18845	4040997..4043168	-	2172	WP_003088105.1	type I secretion system permease/ATPase	-
HUS13_RS18850	4043158..4044435	-	1278	WP_003088104.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-187)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21885.74 Da
• Isoelectric Point: 5.3655

>T160520 WP_003088107.1 NZ_CP054845:4039133-4039711 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDAKTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T160520 NZ_CP070549:3635755-3636129 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT160520 WP_003088112.1 NZ_CP054845:4038490-4039044 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT160520 NZ_CP070549:3635513-3635755 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: V6AG16