Detailed information of TA system TA160515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5664308..5664894
• Replicon: chromosome
• Accession: NZ_CP054844
• Organism: Pseudomonas aeruginosa strain SE5357

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HUS11_RS26995
• Protein ID: WP_003120987.1
• Coordinates: 5664595..5664894 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HUS11_RS26990
• Protein ID: WP_003448662.1
• Coordinates: 5664308..5664598 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS11_RS26975	5659890..5661779	+	1890	WP_042931627.1	hypothetical protein	-
HUS11_RS26980	5661776..5663752	+	1977	WP_042931628.1	DEAD/DEAH box helicase	-
HUS11_RS26985	5663893..5664237	+	345	WP_003448665.1	hypothetical protein	-
HUS11_RS26990	5664308..5664598	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HUS11_RS26995	5664595..5664894	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUS11_RS27000	5665096..5666217	+	1122	WP_003448658.1	TcpQ domain-containing protein	-
HUS11_RS27005	5666217..5667926	+	1710	WP_010792227.1	PilN family type IVB pilus formation outer membrane protein	-
HUS11_RS27010	5667930..5669255	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
HUS11_RS27015	5669245..5669778	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5633067..5721669	88602

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T160515 WP_003120987.1 NZ_CP054844:c5664894-5664595 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T160515 NZ_CP070549:c2540090-2539683 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCCTGTCAGGGTGAGATTCGGCTGTTGATGGATGGCCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCTGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AGAAGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT160515 WP_003448662.1 NZ_CP054844:c5664598-5664308 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT160515 NZ_CP070549:c2540337-2540071 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGAGGTATGCGCGAGCTGGATATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTGAGCTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: G8CP73