Detailed information of TA system TA160514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4849115..4850373
• Replicon: chromosome
• Accession: NZ_CP054844
• Organism: Pseudomonas aeruginosa strain SE5357

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: HUS11_RS22855
• Protein ID: WP_003112528.1
• Coordinates: 4849115..4849870 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HUS11_RS22860
• Protein ID: WP_003086329.1
• Coordinates: 4850005..4850373 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS11_RS22845	4844288..4846831	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
HUS11_RS22850	4846986..4848347	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
HUS11_RS22855	4849115..4849870	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
HUS11_RS22860	4850005..4850373	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HUS11_RS22865	4850446..4851732	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
HUS11_RS22870	4851788..4853377	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
HUS11_RS22875	4853456..4853932	+	477	WP_003112531.1	hypothetical protein	-
HUS11_RS22880	4853935..4854210	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T160514 WP_003112528.1 NZ_CP054844:c4849870-4849115 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T160514 NZ_CP070549:c1287684-1287581 [Citrobacter freundii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT160514 WP_003086329.1 NZ_CP054844:c4850373-4850005 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT160514 NZ_CP070549:1287576-1287719 [Citrobacter freundii]
CACATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7