Detailed information of TA system TA160513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3832993..3834214
• Replicon: chromosome
• Accession: NZ_CP054844
• Organism: Pseudomonas aeruginosa strain SE5357

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: HUS11_RS17955
• Protein ID: WP_003102740.1
• Coordinates: 3833636..3834214 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUS11_RS17950
• Protein ID: WP_003088112.1
• Coordinates: 3832993..3833547 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS11_RS17925	3828561..3829112	+	552	WP_004343115.1	XRE family transcriptional regulator	-
HUS11_RS17930	3829109..3829507	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HUS11_RS17935	3829520..3829843	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
HUS11_RS17940	3830174..3830635	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HUS11_RS17945	3830640..3832835	+	2196	WP_010794059.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HUS11_RS17950	3832993..3833547	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
HUS11_RS17955	3833636..3834214	+	579	WP_003102740.1	HD domain-containing protein	Toxin
HUS11_RS17960	3834323..3835510	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HUS11_RS17965	3835500..3837671	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
HUS11_RS17970	3837661..3838938	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T160513 WP_003102740.1 NZ_CP054844:3833636-3834214 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T160513 NZ_CP070549:c994133-993957 [Citrobacter freundii]
GTGAAGCAAAGTAAGTTTAGACGCTGGCTTGAATCTCAGGGAGTTGCTGTTACAAACGGCTCAAATCATCTGAAACTGAG
ATATCAGGGAAGGCGTAGCGTTATGCCCAGACATCCAGGTGATGAAATAAAAGAAGCCTTGCGAAAAGCGATTATTAAGC
AGCTTGACTTACAGTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT160513 WP_003088112.1 NZ_CP054844:3832993-3833547 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT160513 NZ_CP070549:c993910-993494 [Citrobacter freundii]
ATGCGTTATCCTGTTACTCTAACACCGGCAGTGGAAGGTGGGTTTGTGGTTTCTTTCCCGGATATTCCGGAAGCATTAAC
TCAGGGAAATACTCGCCATGACGCTTTGCAGGCCGCGCAGGCGGCATTGATTACCGCTTTTGAATTCTATTTCGACGACA
ACGAGGCGATTCCACTCCCTTCGGCGGTTAGCGCTGAAGATGACTACGTAGAAATACCGCTGAGCGTTGCGTCTAAGGTT
CTTCTGCTGAATGCCTTCCTTGAATCAAAAATCACCCAGCAGGAGCTGGCGAACAGGATCGGCAGACCTAAGCAGGAGAT
CACCCGTTTATTTGATTTAAAGCACACGACAAAAATTGATGCCGTGCAGATTGCTGCGCGCGCCTTAGGCAAAGAGCTGG
CGTTAACGATGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07