Detailed information of TA system TA160509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1686030..1687725
• Replicon: chromosome
• Accession: NZ_CP054844
• Organism: Pseudomonas aeruginosa strain SE5357

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: HUS11_RS08035
• Protein ID: WP_003112901.1
• Coordinates: 1686030..1686899 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: HUS11_RS08040
• Protein ID: WP_003112902.1
• Coordinates: 1686892..1687725 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS11_RS08010	1682038..1682889	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
HUS11_RS08015	1682930..1683937	+	1008	WP_003112897.1	ABC transporter substrate-binding protein	-
HUS11_RS08020	1683934..1684710	+	777	WP_003112898.1	ABC transporter permease	-
HUS11_RS08025	1684716..1685477	+	762	WP_003112899.1	SDR family oxidoreductase	-
HUS11_RS08030	1685491..1686021	+	531	WP_003112900.1	cupin domain-containing protein	-
HUS11_RS08035	1686030..1686899	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
HUS11_RS08040	1686892..1687725	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
HUS11_RS08045	1687712..1688509	+	798	WP_003112903.1	SDR family oxidoreductase	-
HUS11_RS08050	1688502..1690184	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
HUS11_RS08055	1690195..1690998	+	804	WP_003130116.1	aspartate dehydrogenase	-
HUS11_RS08060	1691010..1692494	+	1485	WP_003453562.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T160509 WP_003112901.1 NZ_CP054844:1686030-1686899 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T160509 NZ_CP070545:102597-103013 [Citrobacter freundii]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCTGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCAGTGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGATGCTGG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT160509 WP_003112902.1 NZ_CP054844:1686892-1687725 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT160509 NZ_CP070545:102370-102600 [Citrobacter freundii]
ATGCGCACAGTTTCTATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCATTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1