Detailed information of TA system TA160482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3591313..3592534
• Replicon: chromosome
• Accession: NZ_CP054794
• Organism: Pseudomonas aeruginosa strain A0002

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUK78_RS16755
• Protein ID: WP_023518577.1
• Coordinates: 3591956..3592534 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUK78_RS16750
• Protein ID: WP_235175809.1
• Coordinates: 3591313..3591867 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK78_RS16725 (HUK78_16720)	3586881..3587432	+	552	WP_003102727.1	XRE family transcriptional regulator	-
HUK78_RS16730 (HUK78_16725)	3587429..3587827	-	399	WP_023082958.1	NADH-quinone oxidoreductase subunit A	-
HUK78_RS16735 (HUK78_16730)	3587840..3588163	-	324	WP_023875494.1	multidrug efflux SMR transporter	-
HUK78_RS16740 (HUK78_16735)	3588494..3588955	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HUK78_RS16745 (HUK78_16740)	3588960..3591155	+	2196	WP_023518578.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HUK78_RS16750 (HUK78_16745)	3591313..3591867	+	555	WP_235175809.1	helix-turn-helix transcriptional regulator	Antitoxin
HUK78_RS16755 (HUK78_16750)	3591956..3592534	+	579	WP_023518577.1	HD domain-containing protein	Toxin
HUK78_RS16760 (HUK78_16755)	3592643..3593830	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HUK78_RS16765 (HUK78_16760)	3593820..3595991	-	2172	WP_009876279.1	type I secretion system permease/ATPase	-
HUK78_RS16770 (HUK78_16765)	3595981..3597258	-	1278	WP_003122808.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-66)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21815.66 Da
• Isoelectric Point: 5.3577

>T160482 WP_023518577.1 NZ_CP054794:3591956-3592534 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLNY
GRRYTDRAPLFKTLRELLDARTRQCLAARRDA

Nucleotide

• Length: 579 bp

>T160482 NZ_CP070528:356419-356787 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20484.66 Da
• Isoelectric Point: 8.1590

>AT160482 WP_235175809.1 NZ_CP054794:3591313-3591867 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQTLLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT160482 NZ_CP070528:356201-356422 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA