Detailed information of TA system TA160477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5189046..5189632
• Replicon: chromosome
• Accession: NZ_CP054793
• Organism: Pseudomonas aeruginosa strain SE5452

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HUK77_RS24075
• Protein ID: WP_003120987.1
• Coordinates: 5189333..5189632 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HUK77_RS24070
• Protein ID: WP_003448662.1
• Coordinates: 5189046..5189336 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK77_RS24050 (HUK77_24050)	5184198..5184425	+	228	WP_235195488.1	cold-shock protein	-
HUK77_RS24055 (HUK77_24055)	5184628..5186517	+	1890	WP_016851610.1	hypothetical protein	-
HUK77_RS24060 (HUK77_24060)	5186514..5188490	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
HUK77_RS24065 (HUK77_24065)	5188631..5188975	+	345	WP_016851612.1	hypothetical protein	-
HUK77_RS24070 (HUK77_24070)	5189046..5189336	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HUK77_RS24075 (HUK77_24075)	5189333..5189632	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK77_RS24080 (HUK77_24080)	5189834..5190958	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
HUK77_RS24085	5191438..5191797	+	360	Protein_4767	secretin N-terminal domain-containing protein	-
HUK77_RS24090	5191943..5192665	+	723	WP_235195489.1	PilN family type IVB pilus formation outer membrane protein	-
HUK77_RS24095 (HUK77_24090)	5192669..5193994	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
HUK77_RS24100 (HUK77_24095)	5193984..5194517	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5164298..5189632	25334

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T160477 WP_003120987.1 NZ_CP054793:c5189632-5189333 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T160477 NZ_CP070524:c818107-817904 [Morganella morganii]
ATGACTAAACTGGACTATCTCATGCGCTTACGTAAGTGCACTTCTATTGAAACACTGGAACGTGTCATTGAGAAGAACAA
ATATGAACTGACTGATGATGAGCTGGAAGTTTTTTATTCAGCAGCCGATCATCGTCTGGCCGAATTAACCATGAATAAAC
TGTATGACAAAATCCCGGCATCTGTCTGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT160477 WP_003448662.1 NZ_CP054793:c5189336-5189046 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT160477 NZ_CP070524:c818530-818171 [Morganella morganii]
ATGGACGAGTATTCTCCACGAAATTACGATATTGCGGTACTGAAGTATCTGTGTGATGAACTTTGCCGTGAAGGCACACA
AACCCTCATCAGAAGCAATAATTATTGGGTTAATGATTTAGCCTCAGATTCAAACTTAAAACTTAACGAATTATTAGGTT
ACATTGCTGACGTGGCCTGGAGTTTTAAGATAAAACATACACAAAATAAGGATCTCATCTCATTTGTCGATGAATACATC
GATGAAACTTACGCGCTGTTTGGTCAGATGGAAGTTTCGTTTAATGACGTTGAGGAATGGAAGAAGATGGCATCACTGGT
AAGCAGCATGCTGACCAGCGAGGGGCATCTCGTCCACTAA

Structures

Toxin

• AlphaFold DB: G8CP73