Detailed information of TA system TA160476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4464370..4464956
• Replicon: chromosome
• Accession: NZ_CP054793
• Organism: Pseudomonas aeruginosa strain SE5452

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HUK77_RS20550
• Protein ID: WP_003120987.1
• Coordinates: 4464657..4464956 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HUK77_RS20545
• Protein ID: WP_003448662.1
• Coordinates: 4464370..4464660 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK77_RS20525 (HUK77_20520)	4459521..4459730	+	210	WP_003105733.1	cold-shock protein	-
HUK77_RS20530 (HUK77_20525)	4459952..4461841	+	1890	WP_016851610.1	hypothetical protein	-
HUK77_RS20535 (HUK77_20530)	4461838..4463814	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
HUK77_RS20540 (HUK77_20535)	4463955..4464299	+	345	WP_016851612.1	hypothetical protein	-
HUK77_RS20545 (HUK77_20540)	4464370..4464660	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HUK77_RS20550 (HUK77_20545)	4464657..4464956	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK77_RS20555 (HUK77_20550)	4465158..4466282	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
HUK77_RS20560 (HUK77_20555)	4466282..4467991	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
HUK77_RS20565 (HUK77_20560)	4467995..4469320	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
HUK77_RS20570 (HUK77_20565)	4469310..4469843	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4437390..4540227	102837

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T160476 WP_003120987.1 NZ_CP054793:c4464956-4464657 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T160476 NZ_CP070522:c33354-33004 [Morganella morganii]
ATGTGGGATGTCATAACAACCGAGCGCTTTGATGAGTGGTTTCTTGCTCAGAGCGATGCTCTCAGGGAGTCTGTCTATGA
GGCTATGGGCGTACTGGAGAAATTCGGCCCGGCTCTAGGTAGACCGTATGTTGATACGCTAAACGATTCTGATTTTGCCA
ATATGAAAGAGCTGCGCATCCAGCATCATGGTAATCCTGTCAGAGCATTTTTTGCGTTTGATCCTTCACGCCGCGCTATC
GTTCTTTGTGCGGGCGATAAAACCGGATTAAACGAAAAGCGCTTTTATAAGGACATGATCAAATTGGCTGATTCGGAATA
CCGTAAACATCTTGCGAAACTGGAGAAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT160476 WP_003448662.1 NZ_CP054793:c4464660-4464370 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT160476 NZ_CP070522:c33002-32703 [Morganella morganii]
ATGGCTACCTACAGAGAACTTCTGGCTAAAGAAAGTCCTGAAATGCAAGCGCGTGTAGCGGAGCGGGTTGAAGAGGCCAG
CCTCCAGATCGCACTAAGCGCTTTGCGTGACGAACTGAATATTTCACAAACAGAGTTAGCTACAATTATGGGAGTAAAAC
AGCCATCGGTTGCCCGGATGGAACAGGCCGACAATGATCCGAGGCTATCAACGTTGAAGCGCTATGTGAAAGCGCTTGGT
GGAGAATTAAGCCTGGATGTGACGCTCCCTACCGGAAAACGTGTTGCTTTCCATCTGTAA

Structures

Toxin

• AlphaFold DB: G8CP73